| 货号 | 14991S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 使用方法 | WB(1:1000) IP (1:50) F (1:100) |
| 供应商 | CST |
| 背景 | OX40 (TNFRSF4, CD134) is a member of the tumor necrosis factor (TNF) receptor superfamily that regulates T cell activity and immune responses. The OX40 protein contains four cysteine rich domains, a transmembrane domain, and a cytoplasmic tail containing a QEE motif (1,2). OX40 is primarily expressed on activated CD4+ and CD8+ T-cells, while the OX40 ligand (OX40L, TNFSF4, CD252) is predominantly expressed on activated antigen presenting cells (3-7). The engagement of OX40 with OX40L leads to the recruitment of TNF receptor-associated factors (TRAFs) and results in the formation of a TCR-independent signaling complex. One component of this complex, PKCθ, activates the NF-κB pathway (2,8). OX40 signaling through Akt can also enhance TCR signaling directly (9). Research studies indicate that the OX40L-OX40 pathway is associated with inflammation and autoimmune diseases (10). Additional research studies show that OX40 agonists augment anti-tumor immunity in several cancer types (11,12). |
| 存放说明 | -20C |
| 计算分子量 | 30 |
Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (left) or treated (right), using OX40L (D6X2D) Rabbit mAb and co-stained with anti-human CD14 antibody. Cells were treated with Human Interleukin-4 (hIL-4) #8919 (20 ng/ml, 7 d) and Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (50 ng/ml, 7 d). During the last 24 hours of treatment, Human Tumor Necrosis Factor-α (hTNF-α) #8902 (20 ng/ml), Human Interleukin-1β (hIL-1β) #8900 (20 ng/ml), and Lipopolysaccharides (LPS) #14011 (1 μg/ml) were added to the cells. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. | |
Western blot analysis of extracts from various cell lines using OX40L (D6X2D) Rabbit mAb (upper) and β-Actin (D6A8) #8457 (lower). |
