| 货号 | 14029S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:100) F (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Flow cytometric analysis of MCF7 cells (blue) and A-431 cells (green) using P-Cadherin (12H6) Mouse mAb. Anti-mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as secondary antibody. | |
Confocal immunofluorescent analysis of A-431 (positive, left) and MCF7 (negative, right) cells using P-Cadherin (12H6) Mouse mAb (green) and β-Actin (13E5) Rabbit mAb #4970 (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). | |
Immunoprecipitation of P-cadherin from A-431 cell extracts using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or P-Cadherin (12H6) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot was performed using P-Cadherin (12H6) Mouse mAb. | |
Western blot analysis of extracts from A-431 (positive) and MCF7 (negative) cells using P-Cadherin (12H6) Mouse mAb (upper) and Pan-Cadherin (28E12) Rabbit mAb #4073 (lower). |
