| 货号 | 13726S |
| 反应种属 | Human/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IF-IC/F |
| 使用方法 | WB(1:1000) F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Constitutively expressed core particle subunits PSMB5, PSMB7, and PSMB6 provide chymotrypsin-like, trypsin-like, and caspase-like activities, respectively (3). In immune cells involved in antigen presentation, these subunits are replaced by highly homologous, induced β-subunits to form the immunoproteasome (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 23, 28 |
Flow cytometric analysis of Raji cells using PMSB8/LMP7 (1A5) Mouse mAb (blue) compared to Mouse (G3A1) mAb IgG1 Isotype Control #5415 (Red). Anti-mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody. | |
Western blot analysis of extracts from various cell lines using PSMB8/LMP7 (1A5) Mouse mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). (The T2 (13) and RKO (8) cell lines are both deficient in PSMB8/LMP8 protein expression). | |
Confocal immunofluorescent analysis of HeLa cells, untreated (upper, left) or treated with hIFN-γ #8901 (100 ng/ml, 72 hr; upper, right), or ACHN cells (lower, left) and RKO cells (lower, right), using PSMB8/LMP7 (1A5) Mouse mAb (green) and β-Actin (13E5) Rabbit mAb #4970 (red). | |
Western blot analysis of extracts from HeLa and SW620 cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 72 hr; +), using PSMB8/LMP7 (1A5) Mouse mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PSMB5 (hPSMB5-Myc/DDK; +) or Myc/DDK-tagged full-length human PSMB8 (hPSMB8-Myc/DDK; +), using PSMB8/LMP7 (1A5) Mouse mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower). |
