| 货号 | 95558S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:1000) |
| 供应商 | CST |
| 背景 | The Rap1 interacting factor 1 (RIF1) was originally identified as a regulator of telomere homeostasis in yeast and mammalian cells (1). Research studies show that RIF1 regulates the timing of eukaryotic DNA replication origin firing through its affect on chromatin architecture (2-4). Additional studies show that RIF1 is essential for regulating the repair of DNA double strand breaks (DSBs). RIF1 is recruited to sites of DSBs by 53BP1 in response to DNA damage, and suppresses 5 end resection to favor the non-homologous end joining (NHEJ) pathway over homologous recombination (HR) repair (5-9). Oct4 and Smad3 modulate Rif1 expression in mouse embryonic stem cells; Rif1 may regulate embryonic stem cell stability during cell proliferation (10). |
| 存放说明 | -20C |
| 计算分子量 | 274 |
Confocal immunofluorescent analysis of MCF7 (positive; left) and U-2 OS (lower expression; right) cells using RIF1 (D2F2M) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Western blot analysis of extracts from various cell lines using RIF1 (D2F2M) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). |
