| 货号 | 2725S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 目标/特异性 | SHIP1 (C15C9) Rabbit mAb detects endogenous levels of total SHIP1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) |
| 供应商 | CST |
| 背景 | SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate (1). SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus (1,2). Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif (2), followed by tyrosine phosphorylation on the NPXY motif (2). The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1 (3-5). Its effect on calcium flux, cell survival, growth, cell cycle arrest and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1021 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6). |
| 存放说明 | -20C |
| 计算分子量 | 145 |
Western blot analysis of extracts from RL and Molt4 cells using SHIP1 (C15C9) Rabbit mAb.Western免疫印迹。用SHIP1 (C15C9) Rabbit mAb研究RL和Molt4 细胞的细胞提取液。 | |
Flow cytometric analysis of Jurkat cells (red) and RL cells (blue) using SHIP1 (C15C9) Rabbit mAb.流式细胞仪分析Jurkat细胞(红色)和RL细胞(蓝色)，所用抗体为 SHIP1 (C15C9) Rabbit mAb。 |
