| 货号 | 60128S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP F |
| 目标/特异性 | Dectin-1 (E1X3Z) Rabbit mAb recognizes endogenous levels of total Dectin-1 protein. |
| 使用方法 | WB(1:1000) IP (1:200) F (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Dectin-1 is a C-type lectin receptor expressed by macrophages, monocytes, dendrtic cells, neutrophils, and a subset of γδ T cells (1,2). Dectin-1 is a glycoprotein with eight different isoforms, generated through alternative splicing (3-5). It plays an important role in anti-fungal immunity by acting as a pattern recognition receptor for β-glucans found on the cell wall of fungi and some bacteria (5,6). Dectin-1 is composed of a short amino-terminal cytoplasmic domain containing an ITAM-like motif, a transmembrane domain, and an extracellular carboxy-terminal C-type lectin domain (5). Dectin-1 recognizes β-glucans through its C-type lectin domain and transduces signals through its ITAM-like motif by recruiting and activating Syk (7,8). Dendritic cells activated through Dectin-1 promote differentiation of Th17 cells by producing IL-6 and IL-23 (9). |
| 存放说明 | -20C |
| 计算分子量 | 14-45 |
| 参考文献 | 1 . Taylor, P.R. et al. (2002) J Immunol 169, 3876-82. 2 . Martin, B. et al. (2009) Immunity 31, 321-30. 3 . Ariizumi, K. et al. (2000) J Biol Chem 275, 20157-67. 4 . Kato, Y. et al. (2006) Biol Pharm Bull 29, 1580-6. 5 . Willment, J.A. et al. (2001) J Biol Chem 276, 43818-23. 6 . Brown, G.D. and Gordon, S. (2001) Nature 413, 36-7. 7 . Rogers, N.C. et al. (2005) Immunity 22, 507-17. 8 . Underhill, D.M. et al. (2005) Blood 106, 2543-50. 9 . LeibundGut-Landmann, S. et al. (2007) Nat Immunol 8, 630-8. |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Dectin-1 (hDectin-1-Myc/DDK; +), using Dectin-1 (E1X3Z) Rabbit mAb. | |
Immunoprecipitation of Dectin-1 from extracts of MUTZ-3 cells differentiated into interstitial dendritic cells (MUTZ-3 IDC) with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (100 ng/ml), Human Interleukin-4 (hIL-4) #8919 (100 ng/ml), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5 ng/ml) for 7 days. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Dectin-1 (E1X3Z) Rabbit mAb. Western blot analysis was performed using Dectin-1 (E1X3Z) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines and MUTZ-3 cells, differentiated into interstitial dendritic cells (MUTZ-3 IDC) with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (100 ng/ml), Human Interleukin-4 (hIL-4) #8919 (100 ng/ml), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5 ng/ml) for 8 days, using Dectin-1 (E1X3Z) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Flow cytometric analysis of Jurkat cells (blue) and MUTZ-3 cells differentiated into interstitial dendritic cells (MUTZ-3 IDC) (green) with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (100 ng/ml), Human Interleukin-4 (hIL-4) #8919 (100 ng/ml), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5 ng/ml) for 7 days compared to concentration matched Rabbit (D1AE) mAb IgG XP® Isotype Control #3900 (red), using Dectin-1 (E1X3Z) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) was used as a secondary antibody. | |
Flow cytometric analysis of human peripheral blood monocytes co-stained with anti-human CD14-APC antibody using Dectin-1 (E1X3Z) Rabbit mAb (right) compared to a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
