| 货号 | 70896S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IHC-P IF-IC F |
| 目标/特异性 | SLP-76 (D1R1A) Rabbit mAb recognizes endogenous levels of total SLP-76 protein. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:200) F (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SH2 domain-containing leukocyte protein of 76 kDa (SLP-76) is a hematopoietic adapter protein that is important in multiple biochemical signaling pathways and necessary for T cell development and activation (1). ZAP-70 phosphorylates SLP-76 and LAT as a result of TCR ligation. SLP-76 has amino-terminal tyrosine residues followed by a proline rich domain and a carboxy-terminal SH2 domain. Phosphorylation of Tyr113 and Tyr128 result in recruitment of the GEF Vav and the adapter protein Nck (2). TCR ligation also leads to phosphorylation of Tyr145, which mediates an association between SLP-76 and Itk, which is accomplished in part via the proline rich domain of SLP-76 and the SH3 domain of ITK (3). Furthermore, the proline rich domain of SLP-76 binds to the SH3 domains of Grb2-like adapter Gads (3,4). In resting cells, SLP-76 is predominantly in the cytosol. Upon TCR ligation, SLP-76 translocates to the plasma membrane and promotes the assembly of a multi-protein signaling complex that includes Vav, Nck, Itk and PLCγ1 (1). The expression of SLP-76 is tightly regulated; the protein is detected at very early stages of thymocyte development, increases as thymocyte maturation progresses, and is reduced as cells mature to CD4+ CD8+ double-positive thymocytes (5). |
| 存放说明 | -20C |
| 计算分子量 | 76 |
| 参考文献 | 1 . Clements, J.L. (2003) Immunol Rev 191, 211-9. 2 . Bubeck Wardenburg, J. et al. (1998) Immunity 9, 607-16. 3 . Bunnell, S.C. et al. (2000) J Biol Chem 275, 2219-30. 4 . Liu, S.K. et al. (1999) Curr Biol 9, 67-75. 5 . Clements, J.L. et al. (1998) J Immunol 161, 3880-9. |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using SLP-76 (D1R1A) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human infiltrating papillary carcinoma of the breast using SLP-76 (D1R1A) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human tonsil using SLP-76 (D1R1A) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded Jurkat (left) and HeLa (right) cell pellets using SLP-76 (D1R1A) Rabbit mAb. | |
Immunoprecipitation of SLP-76 from Jurkat cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SLP-76 (D1R1A) Rabbit mAb. Western blot analysis was performed using SLP-76 (D1R1A) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using SLP-76 (D1R1A) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Flow cytometric analysis of Jurkat cells (green) and HT-29 cells (blue) using SLP-76 (D1R1A) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Confocal immunofluorescent analysis of Jurkat (left) and HeLa (right) cells using SLP-76 (D1R1A) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
