| 货号 | 12286T |
| 同种亚型 | Rabbit |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | Topoisomerase IIα (D10G9) XP® Rabbit mAb recognizes endogenous levels of total topoisomerase IIα protein. |
| 使用方法 | WB(1:1000) IHC-P (1:400) F (1:100) IF-IC (1:1600) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | DNA topoisomerase I and II are nuclear enzymes, and type II consists of two highly homologous isoforms: topoisomerase IIα and IIβ. These enzymes regulate the topology of DNA, maintain genomic integrity, and are essential for processes such as DNA replication, recombination, transcription, and chromosome segregation by allowing DNA strands to pass through each other (1). Topoisomerase I nicks and rejoins one strand of the duplex DNA, while topoisomerase II transiently breaks and closes double-stranded DNA (2). Topoisomerases are very susceptible to various stresses. Acidic pH or oxidative stress can convert topoisomerases to DNA-breaking nucleases, causing genomic instability and cell death. DNA-damaging topoisomerase targeting drugs (e.g., etoposide) also convert topoisomerases to nucleases, and the enzyme is usually trapped as an intermediate, covalently bound to the 5+ end of the cleaved DNA strand(s). Research studies have shown that this intermediate leads to genomic instability and cell death, and thus, agents that target topoisomerases are highly sought after cancer chemotherapeutic drugs (3). Ca2+-regulated phosphorylation of topoisomerase IIα at Ser1106 modulates the activity of this enzyme and its sensitivity to targeting drugs (4). |
| 存放说明 | -20C |
| 计算分子量 | 190 |
| 参考文献 | 1 . Wang, J.C. (2002) Nat. Rev. Mol. Cell. Biol. 3, 430-440. 2 . Pulleyblank, .E. (1997) Science 277, 648-649. 3 . Li, T.K. and Liu, L.F. (2001) Annu. Rev. Pharmacol. Toxicol. 41, 53-77. 4 . Chikamori, K. et al. (2003) J. Biol. Chem. 278, 12696-12702. |
Confocal immunofluorescent analysis of HeLa cells using Topoisomerase IIα (D10G9) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).采用共聚焦免疫荧光术检测HeLa细胞，使用的抗体为Topoisomerase IIα (D10G9) XP® Rabbit mAb (绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记（红色）。 | |
Western blot analysis of extracts from various cell lines using Topoisomerase IIα (D10G9) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot方法检测不同细胞系的提取物，使用的抗体为Topoisomerase IIα (D10G9) XP® Rabbit mAb （上图） 或 β-Actin (D6A8) Rabbit mAb #8457（下图）。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Topoisomerase IIα (D10G9) XP® Rabbit mAb.免疫组化检测石蜡包埋的人乳腺癌，所使用的抗体为Topoisomerase IIα (D10G9) XP® Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Topoisomerase IIα (D10G9) XP® Rabbit mAb.免疫组化检测石蜡包埋的人结肠癌，所使用的抗体为Topoisomerase IIα (D10G9) XP® Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human lymph node using Topoisomerase IIα (D10G9) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).免疫组化分别检测在control peptide（左）或抗原特异性多肽（右）存在的情况下石蜡包埋的人淋巴结，所使用的抗体为Topoisomerase IIα (D10G9) XP® Rabbit mAb。 | |
Flow cytometric analysis of Jurkat cells using Topoisomerase IIα (D10G9) XP® Rabbit mAb compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.流式细胞术检测Jurkat细胞活性，使用的抗体为Topoisomerase IIα (D10G9) XP® Rabbit mAb（蓝色）与使用浓度匹配的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900的相比较（红色）。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412作为二抗使用。 |
