| 货号 | 12231T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Cyclin B1 (D5C10) XP® Rabbit mAb recognizes endogenous levels of total cyclin B1 protein. This antibody also detects a 100 kDa protein of unknown origin in some cell lines. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:200) IF-IC (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) (1,2) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C (3-5). Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis (6-9). While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 (6,10). At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression (11). Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers (12-14).细胞周期蛋白是一类激活特定细胞周期依赖性激酶以推进细胞周期的蛋白质。所有真核细胞要进入细胞周期都是G2/M转换时激活cdc2/cdk1以调控的。该激活作用分成多个步骤开始于调控亚基cdc2/cdk1与cdc2/cdk1结合以形成促有丝分裂因子（MPF）。MPF保持在失活状态直到被cdk活化激酶（CAK）通过cdc2/cdk1磷酸化161位苏氨酸（1,2），cdc25C可以对cdc2/cdk1的14/15位苏氨酸去磷酸化（3-5）。四个细胞周期蛋白B1磷酸化位点（126,128,133和147位丝氨酸）位于胞质滞留信号（CRS）区域，一般认为这四个磷酸化位点可以调控细胞周期蛋白B1在G2/M检验点时期的移位，促进其聚集在细胞核中并起始有丝分裂（6-9）。 |
| 存放说明 | -20C |
| 计算分子量 | 55 |
| 参考文献 | 1 . Lorca, T. et al. (1992) EMBO J 11, 2381-90. 2 . Harper, J.W. and Elledge, S.J. (1998) Genes Dev 12, 285-9. 3 . Norbury, C. et al. (1991) EMBO J 10, 3321-9. 4 . McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85. 5 . Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001. 6 . Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-20. 7 . Li, J. et al. (1997) Proc Natl Acad Sci U S A 94, 502-7. 8 . Takizawa, C.G. and Morgan, D.O. (2000) Curr Opin Cell Biol 12, 658-65. 9 . Santos, S.D. et al. (2012) Cell 149, 1500-13. 10 . Jackman, M. et al. (2003) Nat Cell Biol 5, 143-8. 11 . Gong, D. and Ferrell, J.E. (2010) Mol Biol Cell 21, 3149-61. 12 . Mashal, R.D. et al. (1996) Cancer Res 56, 4159-63. 13 . Kawamoto, H. et al. (1997) Am J Pathol 150, 15-23. 14 . Soria, J.C. et al. (2000) Cancer Res 60, 4000-4. |
Confocal immunofluorescent analysis of HT-29 cells using Cyclin B1 (D5C10) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用细胞周期蛋白 B1 (D5C10) XP®Rabbit mAb(绿色)对HT-29细胞进行激光共聚焦免疫荧光分析。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using Cyclin B1 (D5C10) XP® Rabbit mAb.使用细胞周期蛋白 B1 (D5C10) XP®Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from HT-29 cells, synchronized in S-phase by double thymidine block (2 nM, 16 hr) followed by release into fresh media for the indicated time, using Cyclin B1 (D5C10) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).使用双胸腺嘧啶阻断(2 nM, 16 hr)将HT-29细胞同质在S期然后将细胞放入新鲜培养基一定时间后，使用细胞周期蛋白 B1 (D5C10) XP®Rabbit mAb(上)或β-Actin (D6A8) Rabbit mAb #8457(下) 进行western blot分析， | |
Flow cytometric analysis of Jurkat cells using Cyclin B1 (D5C10) XP® Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 (DNA content). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.使用细胞周期蛋白 B1 (D5C10) XP®兔mAb和碘化丙啶/RNase Staining Solution #4087 (DNA content)对Jurkat细胞进行流式分析。. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412作为二抗。 |
