MOUSE ANTI HUMAN MACROPHAGES:Low Endotoxin【科瑞奥博】

产品中心

当前位置：首页>产品中心

MOUSE ANTI HUMAN MACROPHAGES:Low Endotoxin

货号： MCA874EL 基本售价： 4885.0 元规格： 0.5 mg

产品信息

概述
货号	MCA874EL
克隆号	MAC387
同种亚型	IgG1
反应种属	Human
来源宿主	Mouse
应用	C, F, P

性能
供应商	Bio-Rad Antibodies
溶解方法	Reconstitute with 1.0ml distilled waterReconstitute with 1.0 ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconsti
运输条件
存放说明	Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at -20^oC only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.2 mg, 0.1 mgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 20 µgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.2 mg, 0.1 mgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 20 µgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.2 mg, 0.1 mgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 20 µgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

声明
本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

参考图片

Staining of human peripheral blood granulocytes with FITC conjugated Mouse anti Human macrophages (MCA874F) following permeabilization with Leucoperm (BUF09)

Macrophages stained with Mouse anti Human Macrophages/ Monocytes (MCA874GT) using enhanced DAB in experimental allergic marmoset brain. Mag. X 400

Staining of human peripheral blood granulocytes with RPE conjugated Mouse anti Human Macrophages (MCA874PE) following permeabilization with Leucoperm (BUF09)

Staining of human peripheral blood granulocytes with Mouse anti Human macrophages (MCA874EL) following permeabilization with Leucoperm (BUF09)

Published customer image:
Mouse anti Human macrophages antibody, clone MAC387 used for the identification of canine macrophages in canine skin by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Dogs develop a strong humoral and cellular immune response to bites of Lu. longipalpis sand flies. Dogs (n = 9) were exposed for 10 min to bites of 20 sand flies three times at one week intervals (first exposure, E1; second exposure, E2; third exposure, E3). (A) Weekly measurement of IgG, IgG1 and IgG2 antibody levels in dogs exposed to sand flies. (B) Induration score in a representative dog 48 h after each of three sand fly exposures. The induration score is an arbitrary scale corresponding to the area of induration and redness resulting from multiple bites where 1≤1 cm², 2≤5 cm², 3≤10 cm² and 4>10 cm². (C) Representative H&E staining of biopsies taken from sand fly bite sites prior to exposure (E0) and 48 h after each of three sand fly exposures (E1–E3). Note marked cellular infiltrate within dermis and thickening of epidermis in E3. (D) Immunohistochemical labeling of tissue sections from E3 demonstrating the presence of abundant CD3+T cells (CD3), macrophages (Mac387) and eosinophil granules (Luna stain).

From: Collin N, Gomes R, Teixeira C, Cheng L, Laughinghouse A, et al. (2009)
Sand Fly Salivary Proteins Induce Strong Cellular Immunity in a Natural Reservoir of Visceral Leishmaniasis with Adverse Consequences for Leishmania.
PLoS Pathog 5(5): e1000441.

Published customer image:
Mouse anti Human macrophages antibody, clone MAC387 used for the identification of macrophages in canine skin by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Identification of salivary proteins from Lu. longipalpis that produce a cellular immune response in dogs. (A) A schematic representation of the reverse antigen screening approach based on the intradermal injection of DNA plasmids in dogs previously exposed to sand fly bites (first exposure, E1; second exposure, E2; third exposure, E3). (B–F) Dogs pre-exposed to sand fly bites were challenged intradermally with DNA plasmids and one pair of salivary gland homogenate (SGH) and PBS (positive and negative controls, respectively) and investigated 48 h post-injection. (B) The number of dogs showing local induration and/or erythema at the site of injection for 35 DNA plasmids coding for secreted salivary molecules. Yellow bars highlight the response of dogs to LJM17 and LJL143. (C) Photograph to demonstrate specificity of the cellular reaction to DNA plasmids and SGH. (D) The diameter of erythema in the absence (◇) or presence (◆) of induration for each dog at the site of injection of SGH, PBS, LJL143 and LJM17 (reactive plasmids) and LJL04 and LJM111 (intermediate and non-reactive plasmids, respectively). (E–F) Skin biopsies (6mm) obtained from injection sites were cut in half and processed for histology and RNA extraction. (E) Representative H&E staining and immunohistochemical labeling of dermal T cells (anti-CD3) and macrophages (Mac387) at the injection sites of SGH, LJL143, LJM17 and LJM111. Note marked dermal infiltrates of inflammatory cells characterized as CD3+ T cells and scattered macrophages (Mac387) in the SGH, LJL143 and LJM17. There is no inflammation with LJM111. (F) Reverse-transcriptase quantitative PCR showing the expression levels of IFN-γ, IL-12, IL-4 and TGF-β for LJL143, LJM17, a pair of SGH and control (a mix of PBS and empty plasmid) 48 h post-injection. Error bars represent means±S.E.

From: Collin N, Gomes R, Teixeira C, Cheng L, Laughinghouse A, et al. (2009)
Sand Fly Salivary Proteins Induce Strong Cellular Immunity in a Natural Reservoir of Visceral Leishmaniasis with Adverse Consequences for Leishmania.
PLoS Pathog 5(5): e1000441.

Published customer image:
Mouse anti Human macrophages antibody, clone MAC387 used for the identification of macrophages in canine skin by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Sand fly salivary recombinant proteins produce a DTH response in dogs previously exposed to sand flies. (A) Purity of the recombinant salivary proteins produced by HEK-293F mammalian cells and purified by a HPLC nickel trap column. (B) The diameter of erythema in the absence (◇) or presence (◆) of induration for each dog at the site of injection 48 h after challenge with salivary gland homogenate (SGH), PBS, recombinant proteins rLJL143 and rLJM17 (reactive), rLJM111 (non-reactive) and a non-related tick recombinant protein TB179. (C) Representative H&E staining and immunohistochemical labeling of T cells (anti-CD3) and macrophages (Mac387) at the injection sites of rLJL143, rLJM17 and rLJM111. Note marked dermal infiltrates of inflammatory cells characterized as CD3+ T cells and scattered macrophages (Mac387) with rLJL143 and rLJM17; rLJM111 is negative.

From: Collin N, Gomes R, Teixeira C, Cheng L, Laughinghouse A, et al. (2009)
Sand Fly Salivary Proteins Induce Strong Cellular Immunity in a Natural Reservoir of Visceral Leishmaniasis with Adverse Consequences for Leishmania.
PLoS Pathog 5(5): e1000441.

Published customer image:
Mouse anti Human macrophages antibody, clone MAC387 used for the identification of macrophages in canine skin by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Bites of Lu. longipalpis sand flies induce a strong focal and systemic adaptive cellular immune response in dogs immunized with LJL143 or LJM17. (A–C) Dogs were exposed to uninfected and infected sand flies for 10 min one month after the final immunization with either LJM17, LJL143 or the empty plasmid (control). (A–C) Skin biopsies (6mm) obtained from bite sites 48 h post challenge with 20 and five uninfected and 10 infected sand flies were cut in half and processed for histology and RNA extraction. (A) Representative H&E staining and immunohistochemical labeling of T cells (anti-CD3) and macrophages (Mac387) at the bite sites of 20 uninfected sand flies in LJL143- and LJM17-immunized and control dogs. (B) Reverse-transcriptase quantitative PCR showing the expression levels of IFN-γ, IL-12, IL-4 and TGF-β at the bite sites of 20 or five uninfected sand flies in LJL143- and LJM17-immunized and control dogs (for control dogs RNA was combined from sites of 20 and 5 uninfected sand fly bites). (C) Same as (B) using 10 infected sand flies. Histological sections from bite sites of five uninfected and 10 infected sand flies are provided as Figure S1 and Figure S2, respectively. (D–E) PBMC from LJL143- and LJM17-immunized and control dogs obtained one week after exposure to sand flies. (D) Frequency and mean fluorescence intensity (MFI) of CD3+ T cells following stimulation with medium, rLJL143 or rLJM17. (E) Frequency of CD4+ and CD8+ T cells expressing IFN-γ in PBMC from LJL143- and LJM17-immunized dogs. Error bars represent means±S.E. * P<0.05, ** P<0.01.

From: Collin N, Gomes R, Teixeira C, Cheng L, Laughinghouse A, et al.
(2009) Sand Fly Salivary Proteins Induce Strong Cellular Immunity in a Natural Reservoir of Visceral Leishmaniasis with Adverse Consequences for Leishmania.
PLoS Pathog 5(5): e1000441.

Published customer image:
Mouse anti Human macrophage antibody, clone MAC387 used for the detection of macrophages in rabbit skin by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
A photomontage of wounds and microscopic changes occurring within 24 hours after surgery. When ATP-vesicles are used, granulation starts to appear within 12 hours (A), and H&E staining indicates a rich cellular component (B). Granulation tissue growth continues and covers the whole wound at 24 hours (C). Granulation tissue shows positive anti-MAC387 staining (D), which is further confirmed by CD163 staining (inset) (E). PCNA staining indicates very active proliferation of these cells (F), which is further confirmed by BrdU antibody staining (inset). Wounds treated with Regranex do not display this rapid growth.

From: Howard JD, Sarojini H, Wan R, Chien S (2014)
Rapid Granulation Tissue Regeneration by Intracellular ATP Delivery-A Comparison with Regranex.
PLoS ONE 9(3): e91787.

Published customer image:
Mouse anti Human macrophage antibody, clone MAC387 used for the detection of macrophages in rabbit skin by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Macrophage accumulation in early days. When the wounds are treated with ATP-vesicles, solid granulation occurs at day 3 (B). The growth is filled with macrophages, which occur not only in wound cavity, but also underneath the ear cartilage (D). The wounds treated with Regranex do not have similar growth (A, C).

From: Howard JD, Sarojini H, Wan R, Chien S (2014)
Rapid Granulation Tissue Regeneration by Intracellular ATP Delivery-A Comparison with Regranex.
PLoS ONE 9(3): e91787.

Published customer image:
Mouse anti Human macrophage antibody, clone MAC387 used for the detection of macrophages in rabbit skin by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Comparison of Regranex and ATP-vesicle treated wounds 3 days postoperatively. Rich collections of macrophages are stained dark brown to black by Anti-Mac387 (top panels), and more collagen is shown by van Gieson stain (low panels) in the wounds treated with ATP-vesicles. Regranex treated wounds do not show a similar effect.

From: Howard JD, Sarojini H, Wan R, Chien S (2014)
Rapid Granulation Tissue Regeneration by Intracellular ATP Delivery-A Comparison with Regranex.
PLoS ONE 9(3): e91787.

Published customer image:
Mouse antiHuman macrophages antibody, clone MAC387 used for the demonstration of lesion associated macrophages in Burkholderia pseudomallei infected marmosets by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative H&E and IHC stained tissue sections from the spleen of a marmoset humanely euthanised at 48 h p.c. challenged with 1.85 × 10² ± 57 cfu of Burkholderia pseudomallei by the subcutaneous route. (a) H & E showing multifocal lesion with severe necrosis (++++), (b) Burkholderia pseudomallei antigen IHC showing abundant bacteria associated with the lesion (++++), (c) IHC staining showing a moderate number of macrophages associated with the lesion (+++), (d) IHC staining showing very few T cells associated with the lesion (+), (e) IHC staining showing very few B cells associated with the lesion (+), (f) IHC staining showing very few inducible Nitric oxide synthase (iNOS) producing cells associated with the lesion (+).

From: Nelson M, Salguero FJ, Dean RE, Ngugi SA, Smither SJ, Atkins TP, Lever MS.
Comparative experimental subcutaneous glanders and melioidosis in the common marmoset (Callithrix jacchus).
Int J Exp Pathol. 2014 Dec;95(6):378-91.
Contains public sector information licensed under the Open Government Licence v3.0.

Published customer image:
Mouse antiHuman macrophages antibody, clone MAC387 used for the demonstration of lesion associated macrophages in Burkholderia pseudomallei infected marmosets by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative H&E and IHC stained tissue sections from the inoculation site of a marmoset humanely euthanised at 36 h p.c. challenged with 1.85 × 10² ± 57 cfu of Burkholderia pseudomallei by the subcutaneous route. (a) H & E showing multifocal lesion with severe necrosis (++++), (b) B. pseudomallei antigen IHC showing abundant bacteria associated with the lesion (++++), (c) IHC staining showing a small number of macrophages associated with the lesion (++), (d) IHC staining showing very few T cells associated with the lesion (+), (e) IHC staining showing no B cells associated with the lesion, (f) IHC staining showing very few inducible Nitric oxide synthase (iNOS) producing cells associated with the lesion (+).

From: Nelson M, Salguero FJ, Dean RE, Ngugi SA, Smither SJ, Atkins TP, Lever MS.
Comparative experimental subcutaneous glanders and melioidosis in the common marmoset (Callithrix jacchus).
Int J Exp Pathol. 2014 Dec;95(6):378-91.
Contains public sector information licensed under the Open Government Licence v3.0.