MOUSE ANTI HUMAN CD54:Low Endotoxin【科瑞奥博】

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MOUSE ANTI HUMAN CD54:Low Endotoxin

货号： MCA1615EL 基本售价： 4885.0 元规格： 0.5 mg

产品信息

概述
货号	MCA1615EL
克隆号	15.2
同种亚型	IgG1
反应种属	Human
来源宿主	Mouse
应用	C, F, FN, IP

性能
供应商	Bio-Rad Antibodies
溶解方法	Pack Size: 100 TestsReconstitute with 1 ml distilled waterPack Size: 25 TestsReconstitute in 0.25 ml disilled water
运输条件
存放说明	Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at -20^oC only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

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参考图片

Published customer image:
Mouse anti Human CD54 antibody, clone 15.2 used to demonstrate co-localization of endogenous ICAM-1 with ICAM-1 GFP in human endothelial cells by immunofluorescence.
Image caption:
ICAM-1-GFP co-localizes with endogenous ICAM-1 and associates to filamin and actin upon clustering. (A) TNF-α-stimulated endothelium was transiently transfected with ICAM-1-GFP, fixed and stained for ICAM-1-GFP in green, endogenous ICAM-1 in red. Merge shows co-localization in yellow and F-actin is in grayscale. Images were taken from the apical surface (upper panel) and at the baso-lateral plane (lower panel). Bars, 50 μm. (B) Beads, coated with ICAM-1 Ab, were allowed to adhere for 30 minutes to TNF-α-stimulated HUVECs. ICAM-1 was stained using an ICAM-1 Ab that is directly labelled with ALEXA 647. The results show that ICAM-1-GFP (green) and endogenous ICAM-1 (red) co-localize (Arrowheads; Merge in yellow) at sites of bead adhesion. DIC image shows localization of the beads. Images were taken from the apical surface (upper panel) and at the baso-lateral plane (lower panel). Bars, 10 μm. (C) ICAM-1-GFP was transfected into HeLa cells and anti-ICAM-1-coated magnetic beads were allowed to adhere for 30 minutes, resulting in clustering of ICAM-1, after which the cells were lysed ands treated as described in the legend of figure 2C. The images on the left show that the magnetic beads efficiently precipitated ICAM-1-GFP and that ICAM-1 clustering resulted in the recruitment of actin and filamin A. Images on the right show the expression of indicated proteins in the cell lysates. (D) ICAM-1-GFP was transfected into HeLa cells and anti-ICAM-1-coated magnetic beads were allowed to adhere for 5 or 20 minutes, as indicated. ICAM-1 was precipitated at both time points, whereas actin and filamin A and B were associated to ICAM-1 only after 20 minutes of clustering. Images on the right show the expression of indicated proteins in the cell lysates.

From: van Buul JD, van Rijssel J, van Alphen FPJ, Hoogenboezem M, Tol S, Hoeben KA, et al. (2010)
Inside-Out Regulation of ICAM-1 Dynamics in TNF-α-Activated Endothelium.
PLoS ONE 5(6): e11336.

Published customer image:
Schematic representation of ligand binding sites on CD54 and epitope representation for Mouse anti Human CD54 antibody, clone 15.2. Effect of blocking of ICAM-1: ligand interaction by Mouse anti Human CD54 antibody, clone 15.2 on Ifn-γ production by NK cells in vitro.
Image caption:
Engagement of ICAM-1 with its cellular ligand but not with PfEMP1 is required for NK cell IFN-γ production. (A) Diagram of an ICAM-1 molecule showing schematic binding sites for LFA-1, Mac-1, CD11c/CD18 and PfEMP1. The epitope map of the anti-ICAM-1 mAb 15.2, My13 and RR1/1 is indicated. (B) Human PBMC were cultured with uninfected RBC (RBC, black bars) or with RBC infected with the 3D7 Pf strain (3D7, grey bars) in presence or absence of antibodies directed against NKG2D (isotype control), ICAM-1 or CD18. Three different clones of anti-ICAM-1 were used: 15.2 blocks the interaction of ICAM-1 with LFA-1 and with PfEMP1, RR1/1 blocks only the interaction with LFA-1 and My13 blocks only the interaction with PfEMP1. After 24 h of co-culture, NK cell activation was analyzed by flow cytometry by gating on CD3-CD56⁺ NK cells. The CD69 MFI staining on NK cells (left panel), the percentage of CD25⁺ NK cells (middle panel) and the percentage of IFN-γ⁺ NK cells (right panel) were determined for 24 donors (None, NKG2D and 15.2), 13 donors (CD18), 9 donors (My13) or 5 donors (RR1/1). Means ± SEM are represented. Statistical analyses were performed using the Mann Whitney test.

From: Baratin M, Roetynck S, Pouvelle B, Lemmers C, Viebig NK, Johansson S, et al. (2007)
Dissection of the Role of PfEMP1 and ICAM-1 in the Sensing of Plasmodium falciparum-Infected Erythrocytes by Natural Killer Cells.
PLoS ONE 2(2): e228.

Published customer image:
Mouse anti Human CD54 antibody, clone 15.2 used for the evaluation of ICAM-1 (CD54) expression on NK cells of differing origins by flow cytometry.
Image caption:
Primary resting human NK cells (CD56+CD3-) in total PBMC as well as human NK cell lines, NK92 and NKL were analyzed by flow cytometry to determine the surface expression of three host ligands for PfEMP1: CSA (left panels, dark line), CD36 (middle panels, dark line) and ICAM-1 (right panels, dark line). Stainings with isotype control for each antibody are represented by filled grey histograms. Stainings of primary resting human NK cells are representative of at least 3 donors.

From: Baratin M, Roetynck S, Pouvelle B, Lemmers C, Viebig NK, Johansson S, et al. (2007)
Dissection of the Role of PfEMP1 and ICAM-1 in the Sensing of Plasmodium falciparum-Infected Erythrocytes by Natural Killer Cells.
PLoS ONE 2(2): e228.

Figure A. FITC conjugated mouse anti human CD40 (MCA1590PE) and Alexa647® conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated mouse anti human CD40 (MCA1590PE) and Alexa647® conjugated mouse anti human CD54 (MCA1615A647). All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).

Figure A. FITC conjugated mouse anti human CD19 (MCA2495F) and Alexa647® conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated mouse anti human CD19 (MCA2495F) and Alexa647® conjugated mouse anti human CD54 (MCA1615A647). All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).