MOUSE ANTI HUMAN CD83:RPE【科瑞奥博】

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MOUSE ANTI HUMAN CD83:RPE

货号： MCA1582PE 基本售价： 3985.0 元规格： 100 TESTS

产品信息

概述
货号	MCA1582PE
克隆号	HB15e
同种亚型	IgG1
反应种属	Human
来源宿主	Mouse
应用	F

性能
供应商	Bio-Rad Antibodies
溶解方法	Pack Size: 100 TestsReconstitute with 1.0 ml distilled waterPack Size: 25 TestsReconstitute in 0.25 ml disilled water
运输条件
存放说明	Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

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参考图片

Staining of KM-H2 cell line with Mouse anti Human CD83:FITC (MCA1582F)

Staining of KM-H2 cells with Mouse anti Human CD83 (MCA1582)

Staining of KM-H2 cells with Mouse anti Human CD83:Alexa Fluor^® 647 (MCA1582A647)

Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e (MCA1582) followed by the Histar detection system (STAR 3000). Low power

Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e (MCA1582) followed by the Histar detection system (STAR 3000). Medium power

Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e (MCA1582) followed by the Histar detection system (STAR 3000). High power

Immunofluorescence staining of a human tonsil cryostection with Mouse anti Human CD83 antibody, clone HB15e (MCA1582), red in A and Mouse anti Human CD21 antibody, clone LB21 (MCA1195), green in B. The merged image is in C with nuclei counterstained blue using DAPI. Low power

Published customer image:
Phenotypic characterization of immunogenic and tolerogenic moDC populations by flow cytometry. Monocytes were negatively selected from PBMC using magnetic beads. Immature moDC were generated with IL-4 and GM-CSF for 6 days. 15d-PGJ2 (PGJ2 DC) and dexamethasone plus 1a,25-dihydroxyvitamin were added to generate tolerogenic moDC, respectively (PGJ2 DC and Dex/VD3 DC). To generate immunogenic moDC, immature moDC were stimulated for 24 h with LPS, polyI:C and a cytokine cocktail containing TNF-a, IL-1ß, IL-6 and PGE2, respectively. The phenotypes of the cells were analyzed by flow cytometry. Live cells were gated according to FSC/SSC. One representative experiment out of three is shown.

From: Sprater F, Hovden A-O, Appel S (2012) Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells. PLoS ONE 7(11): e49577.