货号 | AHP394 |
同种亚型 | Polyclonal IgG |
反应种属 | Human |
来源宿主 | Sheep |
应用 | E, GS, IP, WB |
供应商 | Bio-Rad Antibodies |
运输条件 | |
存放说明 | Store at +4oC for one month or at -20oC for longer. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
本官网所有报价均为常温或者蓝冰运输价格,如有产品需要干冰运输,需另外加收干冰运输费。 |
Western blot analysis of NFkB IP samples. IP was performed on Jurkat cell lysates using 10 µg (lanes 2 & 7), 5 µg (lanes 3 & 8), 1 µg (lanes 4 & 9) Sheep anti NFkB p65 antibody (AHP394), and 10 µg polyclonal IgG (lanes 5 & 10). 8.75 µl of each IP was loaded onto an AnykD ™ Criterion ™ TGX Stain-Free™ gel. Precision Plus Protein™ Prestained Standards were run lanes 1 & 6. Mouse anti NFkB p65 (MCA6021) was used at 1/1000 in lanes 1-5. HRP conjugated Goat anti Mouse IgG (H/L) antibody (STAR117P) was used at 1/10,000 and visualized on the Bio-Rad Chemidoc™ Touch Imaging System. Arrow points to NFkB (molecular weight 65 kDa). |