| 货号 | MCA532 |
| 克隆号 | 84H10 |
| 同种亚型 | IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | C*, E, F, FN*, IP |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Published customer image: ICAM-1 activates PKCa but not PKCßII in HMVECLs. A ) At 70% confluence, HMVECLs cells pretreated with 10 ng/ml TNFa to induce ICAM-1 expression. At 24 hrs, the cells were suspended and immunolabeled with anti-ICAM-1 antibodies and examined by flow cytometry for ICAM-1 expression. B–D) HMVECLs were pretreated with TNFa as in panel A. At 24 hrs, the endothelial cells were nonstimulated (NS) or stimulated with a confluent monolayer of anti-ICAM-1-coated beads or the control anti-PECAM-1-coated beads for 20 minutes in B and D or for the times indicated in C. The cells were lysed and the activation of PKCßII and PKCa was examined by western blot with B) anti-phospho-PKCßIIThr641 or anti-PKCßII or C–D) anti-phospho- PKCaThr638 or anti-PKCa. Shown are representative blots. Shown are the mean ± SEM of 3 experiments. NT, nontreated. *, p<0.05 as compared to the nontreated (NT) groups. From: Abdala-Valencia H, Berdnikovs S, Cook-Mills JM (2012) Vitamin E Isoforms Differentially Regulate Intercellular Adhesion Molecule-1 Activation of PKCa in Human Microvascular Endothelial Cells. PLoS ONE 7(7): e41054. |
