| 货号 | MCA1539 |
| 克隆号 | LOB 3/17 |
| 同种亚型 | IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | F, IP |
| 供应商 | Bio-Rad Antibodies |
| 溶解方法 | Reconstitute in 0.25 ml disilled water |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4oC. Following reconstitution store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Staining of HUT78 T cell line with Mouse anti Human CD95:FITC (MCA1539F) | |
Staining of human peripheral blood granulocytes with Mouse anti Human CD95:Alexa Fluor® 647 (MCA1539A647) | |
Published customer image: Mouse anti Human CD95 antibody, clone LOB 3/17 (MCA1539) used for the evaluation of CD95 expression on HUT78 T cell lymphoma cells by flow cytometry Image caption: Soluble CD154 inhibits Fas-mediated cell death in HUT78 T cells. (A, Left and middle pannels) HUT78 cells were incubated with anti-α5β1 JBS5 mAb, anti-CD40 G28.5 mAb, anti-Fas LOB3/17 mAb, or IgG isotype control (IC) followed by mouse anti-human Ig labeled with Alexa 488. (A, Right panel) Cells were incubated with Avidin-Alexa 488 (negative control) or sCD154-Alexa 488 (20ng/sample). (B, Left panel) HUT78 T cells were left non-stimulated (NS) or stimulated with different concentrations of anti-Fas CH-11 mAb or IgM isotype control (IC) for 48 hours at 37°C and assessed for their cell death response by FACS analysis as the percentage of death obtained by propidium iodide staining. (B, Right panel) HUT78 cells were pre-treated with different concentrations of sCD154, then left non-stimulated (NS) or stimulated with the anti-Fas CH-11 mAb (200ng/ml) for 48 h at 37°C. Cell death was then evaluated as above (± SD, * p <0.05, ** p <0.01, *** p <0.001 CH-11 versus IC). From: Bachsais M, Naddaf N, Yacoub D, Salti S, Alaaeddine N, Aoudjit F, et al. (2016) The Interaction of CD154 with the α5β1 Integrin Inhibits Fas-Induced T Cell Death. PLoS ONE 11(7): e0158987. |
