| 货号 | MCA2588A488 |
| 克隆号 | Y2/51 |
| 同种亚型 | IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | F |
| 供应商 | Bio-Rad Antibodies |
| 溶解方法 | Reconstitute with 1.0ml distilled water |
| 运输条件 | |
| 存放说明 | Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Published customer image: Alexafluor 647 conjugated Mouse anti Human CD61 antibody, clone Y2/51 (MCA2588A647) used for the assessment of CD61 expression by platelets by immunofluorescence. Image caption: Minocycline reduces activation of human platelets in vitro. Platelets from healthy, seronegative donors were isolated and activation was assessed via (A) measurement of sCD40L levels by ELISA after treatment with thrombin alone or together with minocycline, or (B) measurement of CD62P (P selectin; a platelet activation marker) median fluorescent intensity (MFI) by flow cytometry. Data from (B) is presented as fold change of CD62P MFI normalized to non-treated (NT) samples (visualized by horizontal black line set at 1). Data sets for (A) were compared using a one way ANOVA statistical test and data sets for (B) were compared using a two way ANOVA statistical test followed by Bonferroni's test for multiple comparisons, where * denotes p<0.05. Data shown is representative of three separate experiments. (C) Platelet degranulation was assessed via measurement of mepacrine retention by flow cytometry. The data is representative of three separate experiments and was compared using a one way ANOVA statistical test followed by Bonferroni's test for multiple comparisons, where * denotes p<0.05. (D) Representative images of mepacrine retention in platelet granules as analyzed by Amnis Image Stream following the indicated treatments. CD61 marks the platelet surface (in red), and mepacrine is stored in platelet dense granules (in green). From: Jackson JW, Singh MV, Singh VB, Jones LD, Davidson GA, Ture S, et al. (2016) Novel Antiplatelet Activity of Minocycline Involves Inhibition of MLK3-p38 Mitogen Activated Protein Kinase Axis. PLoS ONE 11(6): e0157115. | |
Peripheral Human Monocytes stained with Mouse anti Human CD61:RPE (MCA2588PE) |
