| 货号 | MCA2143 |
| 克隆号 | DAXX-01 |
| 同种亚型 | IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | C, WB |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Published customer image: Mouse anti Human DAXX antibody, clone DAXX-01 (MCA2143) used for the detection of DAXX in iSLK cells by immunofluorescence and western blotting. Image caption: KSHV infection antagonizes the ND10 component ATRX. iSLK cells were seeded onto glass slides, infected with KSHV strain rKSHV.219 for 24A: single staining for PML (c), Sp100 (g) and ATRX (l) B: double staining for ATRX (c) and Daxx (d). Arrows indicate infected cells. C: iSLK cells were infected with KSHV strain rKSHV.219 with high MOI and harvested 20 h post infection. Whole cell lysates were prepared and expression of ND10 components analyzed by immunoblotting. From: Full F, Jungnickl D, Reuter N, Bogner E, Brulois K, Scholz B, et al. (2014) Kaposi's Sarcoma Associated Herpesvirus Tegument Protein ORF75 Is Essential for Viral Lytic Replication and Plays a Critical Role in the Antagonization of ND10-Instituted Intrinsic Immunity. PLoS Pathog 10(1): e1003863. | |
Published customer image: Mouse anti Human DAXX antibody, clone DAXX-01 (MCA2143) used for the detection of DAXX in human foreskin fibroblasts by western blotting. Image caption: KSHV ORF75 is sufficient for antagonization of ATRX and Sp100. A: HFF cells were transfected with a pcDNA-orf75-myc expression construct. Cells were analyzed by immunofluorescence with a confocal microscope, and at least 50 individual cells per each box analyzed by Fiji. Statistical analyses were conducted using the unpaired t test for SP100 and PML, and one way ANOVA test for multiple comparisons for Daxx and ATRX, respectively, of GraphPad Prism 6 for Windows. **p<0.01; *** p<0.001. B: 293T cells were transfected with increasing amounts of ORF75 and endogenous expression of ATRX and Daxx was analyzed by immunoblot. C: 293T cells were cotransfected with constant levels of Sp100 and increasing amounts of ORF75 and expression of Sp100 and Daxx levels analyzed by immunoblot. From: Full F, Jungnickl D, Reuter N, Bogner E, Brulois K, Scholz B, et al. (2014) Kaposi's Sarcoma Associated Herpesvirus Tegument Protein ORF75 Is Essential for Viral Lytic Replication and Plays a Critical Role in the Antagonization of ND10-Instituted Intrinsic Immunity. PLoS Pathog 10(1): e1003863. | |
Published customer image: Mouse anti Human DAXX antibody, clone DAXX-01 (MCA2143) used for the detection of DAXX in human foreskin fibroblasts by immunofluorescence. Image caption: Diffuse localization of ATRX after knockdown of Daxx. HFF cells carrying retroviral knockdown shRNA vectors targeting Daxx or PML were immunostained with respective antibodies. ND10 accumulation as shown by colocalization (c) with PML (a) of ATRX (b) is lost in Daxx-kd cells (e,f) while PML (d) remains in ND-10 structures. In contrast, knockdown of PML (g–i) results in dispersal of ND10 and seemingly a partial colocalization (i) of Daxx (g) and ATRX (h) into smaller structures; most of Daxx and ATRX proteins are not colocalized. From: Full F, Jungnickl D, Reuter N, Bogner E, Brulois K, Scholz B, et al. (2014) Kaposi's Sarcoma Associated Herpesvirus Tegument Protein ORF75 Is Essential for Viral Lytic Replication and Plays a Critical Role in the Antagonization of ND10-Instituted Intrinsic Immunity. PLoS Pathog 10(1): e1003863. |
