| 货号 | 7440-0104F |
| 同种亚型 | Polyclonal IgG |
| 反应种属 | Human |
| 来源宿主 | Sheep |
| 应用 | IF |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Published customer image: Sheep anti Human plasminogen antibody used to detect binding of human plasminogen to the outer envelope of Francisella tularensis by immunogluorescence. Image caption: PLG binds to the outer envelope of FT. Laser scanning confocal microscopy of PLG-associated FTLVS. Bound huPLG ligand was detected using sheep anti-human PLG antibody followed by incubation with Dylight-488 conjugated donkey, anti-sheep/goat IgG secondary antibody. Samples were visualized using a Zeiss LSM 510 confocal microscope. From: Clinton SR, Bina JE, Hatch TP, Whitt MA, Miller MA. Binding and activation of host plasminogen on the surface of Francisella tularensis. BMC Microbiol. 2010 Mar 12;10:76. | |
Published customer image: Sheep anti Human plasminogen antibody used to detect binding of human plasminogen to the outer envelope of Francisella tularensis by western blotting. Image caption: Identification of putative PLG-binding proteins of FT. Sarkosyl-soluble and insoluble protein fractions of FTLVS were separated by SDS-PAGE and transferred to PVDF membrane. Membranes were then blotted with huPLG (3 ug/mL) followed by anti-PLG antibody and HRP-conjugated secondary antibody to detect PLG-binding proteins (Panel A). Protein bands on an identical Coomassie Blue-stained SDS-PAGE gel corresponding to those identified via blotting (Panel B) were excised and identified using proteomic methodologies (Panel C). BMC Microbiol. 2010 Mar 12;10:76. | |
Published customer image: Sheep anti Human plasminogen antibody used for the detection of plasminogen in Jurkat cell lysates by western blotting. Image caption: Necrotic cells harbour misfolded proteins and generate surface-bound plasmin which increases their phagocytosis by human MoDCs. (A) Uninjured and necrotic Jurkat lymphocytes were stained with 10 mg/L 7AAD and 10 mg /L Thiazine Red for 15 min then subjected to flow cytometry. (B) The Triton-insoluble fractions of uninjured and necrotic Jurkat lymphocytes were subjected to SDS-PAGE under reducing conditions and subsequent Coomassie staining. (C) Uninjured and necrotic Jurkat lymphocytes were incubated with t-PA and/or plasminogen for 15 min and washed. Total cellular protein lysates were prepared and subjected to SDS-PAGE under reducing conditions and subsequent immunoblot analysis. Most of the t-PA used was in single-chain form (~70 kDa), however a small extent of two-chain t-PA was also apparent (the band indicated by the asterisk at ~35 kDa). (D) PKH67-labelled necrotic Jurkat lymphocytes were treated with the indicated reagents for 15 min, then washed and incubated with PKH26-labelled human MoDCs. 24 h later, the proportion of double-positive (PKH67positive and PKH26positive) MoDCs was assessed by flow cytometry. Data are displayed as fold-change in double-positive MoDCs (mean ± s.e.m.; n = 3–5 independent experiments). Data was normalized to the group where necrotic cells received no exogenous reagent. **p<0.01 and ***p<0.001 by 1-way ANOVA with Newman-Keuls post-hoc analysis. From: Borg RJ, Samson AL, Au AE-L, Scholzen A, Fuchsberger M, Kong YY, et al. (2015) Dendritic Cell-Mediated Phagocytosis but Not Immune Activation Is Enhanced by Plasmin. PLoS ONE 10(7): e0131216. |
