| 货号 | MCA2279GT |
| 克隆号 | 57/3 |
| 同种亚型 | IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | P* |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta 2 antibody (MCA2279) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power) | |
Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta 2 antibody (MCA2279) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (high power) | |
Published customer image: Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons. All proteins were immunolocalised to cell nuclei (see higher power inserts in panels e, f, c and d respectively). In the well and moderately differentiated cancers expression was most intense in epithelial cell layers (arrowheads, panel A and inserts). Note that ERa was low/absent in poor grade cancers (J) but immunoexpression of ERß1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERß1, ERß2 and ERß5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers. From: Collins F, MacPherson S, Brown P, Bombail V, Williams AR, Anderson RA, Jabbour HN, Saunders PT. Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha. BMC Cancer. 2009 Sep 16;9:330. | |
Published customer image: Mouse anti Human estrogen receptor β2 used for the detection of ERβ2 expressing cells in tumor tissue by immunohistochemistry in formalin fixed, paraffin embedded tissue sections. Image caption: Effect of gender on ERß1, ERß2 and ERß5 expression in D283 Med xenografts. A) Immunostaining for ERß1 was significantly higher in tumors from females compared to males (**p<0.01); B) and C) Tumor levels of ERß2 and ERß5 did not significantly differ between females and males. All results are expressed as the mean ± SEM (n = 6 tumors/group). Representative stained section of tumors from females and males are shown (magnification 40x). IHC, immunohistochemical score. From: Ciucci A, Meco D, De Stefano I, Travaglia D, Zannoni GF, et al. (2014) Gender Effect in Experimental Models of Human Medulloblastoma: Does the Estrogen Receptor ß Signaling Play a Role? PLoS ONE 9(7): e101623. | |
Published customer image: Mouse anti Human estrogen receptor β2 used for the detection of ERβ2 expressing cells in normal human liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Image caption: Staining pattern of the ERß isoforms in human liver. In order to confirm the specificity of the ERß antibodies used in the study, we immunostained sections of human healthy liver, showing that ERß1 and ERß2 isoforms are not expressed, while specific nuclear ERß5 immunoreactivity is detected (magnification 20x and 40x). This pattern of ERß isoforms immunoreactivity is consistent with previous descriptions. From: Ciucci A, Meco D, De Stefano I, Travaglia D, Zannoni GF, et al. (2014) Gender Effect in Experimental Models of Human Medulloblastoma: Does the Estrogen Receptor ß Signaling Play a Role? PLoS ONE 9(7): e101623. |
