Human ULBP-2/5/6 PerCP MAb (Clone 165903) (100 TESTS)
货号：
FAB1298C 基本售价：
5661.2 元 规格：
100 Tests
产品信息
概述| 货号 | FAB1298C |
| 别名 | ULBP-2/5/6 |
全称 | UL16 Binding Protein-2/5/6 | | 反应种属 | Human |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross-reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry. |
| 使用方法 | Flow Cytometry: 10 µL/106cells
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | BaF3 mouse pro-B cell line transfected with human ULBP-2
Accession # Q9BZM5 |
| 生物活性 | Human |
| 标记 | PerCP |
| 背景 | ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Cosman, D. et al. (2001) Immunity 14:123.
- Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
- Sutherland, C. et al. (2002) J. Immunol. 168:671.
- Steinle, A. et al. (2001) Immunogenetics 53:279.
- Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
- Pende, D. et al. (2002) Cancer Res. 62:6178.
- Radosavljevic, M. et al. (2002) Genomics 79:114.
- NKG2D and its Ligands (2002) www.RnDSystems.com.
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参考图片Detection of ULBP-2/5/6 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB1298C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins. |
