Human ULBP-2/5/6 Allophycocyanin MAb (Clone 165903) (100 TESTS)【科瑞奥博】

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Human ULBP-2/5/6 Allophycocyanin MAb (Clone 165903) (100 TESTS)

货号： FAB1298A 基本售价： 5512.4 元规格： 100 Tests

产品信息

概述
货号	FAB1298A
别名	ULBP-2/5/6
全称	UL16 Binding Protein-2/5/6
反应种属	Human
应用	Flow Cytometry
目标/特异性	Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross‑reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. PRL-3 mediates the protein maturation of ULBP2 by regulating the tyrosine phosphorylation of HSP60. Authors: Leung W, Vong Q, Lin W, Bouck D, Wendt S, Sullivan E, Li Y, Bari R, Chen T, Leung W J Immunol, 2015;194(6):2930-41. Species: Human Sample Type: Whole Cells Application: Flow The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway. Authors: Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S J Immunol, 2014;193(4):1654-65. Species: Human Sample Type: Whole Cells Application: Flow Modulation of NKG2D ligand expression and metastasis in tumors by spironolactone via RXRgamma activation. Authors: Leung W, Vong Q, Lin W, Janke L, Chen T, Leung W J Exp Med, 2013;210(12):2675-92. Species: Human Sample Type: Whole Cells Application: Flow Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44. Authors: Madrid, Alexis S, Ganem, Don J Virol, 2012;86(16):8693-704. Species: Human Sample Type: Whole Cells Application: Flow Vesicular Stomatitis Virus Infection Promotes Immune Evasion by Preventing NKG2D-Ligand Surface Expression. Authors: Jensen H, Andresen L, Nielsen J, Christensen JP, Skov S PLoS ONE, 2011;6(8):e23023. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	BaF3 mouse pro-B cell line transfected with human ULBP-2 Accession # Q9BZM5
生物活性	Human
标记	Allophycocyanin
背景	ULBPs activatemultiple signaling pathways in primary NK cells, resulting in the production ofcytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calciummobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signaltransduction pathway. The name ULBP derives from the original identification ofthree proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirusglycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genesfor ULBPs reside in a cluster of ten related genes, six of which encodepotentially functional glycoproteins. ULBP-2 has also been described under thenames RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins aredistantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin.Some family members, including ULBP-2, are anchored to the membrane via aGPI-linkage, whereas others have transmembrane domains. Engagement of NKG2Dresults in the activation of cytolytic activity and/or cytokine production bythese effector cells. The ULBPs are expressed on some tumor cells and have beenimplicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cosman, D. et al. (2001) Immunity 14:123. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428. Sutherland, C. et al. (2002) J. Immunol. 168:671. Steinle, A. et al. (2001) Immunogenetics 53:279. Sutherland, C. et al. (2001) Immunol. Rev. 181:185. Pende, D. et al. (2002) Cancer Res. 62:6178. Radosavljevic, M. et al. (2002) Genomics 79:114. NKG2D and its Ligands (2002) www.RnDSystems.com.

参考图片

Detection of ULBP-2/5/6 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 APC‑conjugated Monoclonal Antibody (Catalog # FAB1298A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.