Human TIM-3 Allophycocyanin MAb (Clone 344823) (100 TESTS)【科瑞奥博】

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Human TIM-3 Allophycocyanin MAb (Clone 344823) (100 TESTS)

货号： FAB2365A 基本售价： 5373.6 元规格： 100 Tests

产品信息

概述
货号	FAB2365A
简介	Clone 344823 was used by HLDA to establish CD designation
别名	CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; Tim-3; TIMD-3; TIMD3KIM-3
全称	T Cell Immunoglobulin Mucin-3
反应种属	Human
应用	Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	84868 (Human); 171285 (Mouse); 102141722 (Cynomolgus Monkey)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TIM-3/Gal-9 interaction induces IFNgamma-dependent IDO1 expression in acute myeloid leukemia blast cells. Authors: Folgiero V, Cifaldi L, Li Pira G, Goffredo B, Vinti L, Locatelli F J Hematol Oncol, 2015;8(0):36. Species: Human Sample Type: Whole Cells Application: Flow Inhibitory phenotype of HBV-specific CD4+ T-cells is characterized by high PD-1 expression but absent coregulation of multiple inhibitory molecules. Authors: Raziorrouh B, Heeg M, Kurktschiev P, Schraut W, Zachoval R, Wendtner C, Wachtler M, Spannagl M, Denk G, Ulsenheimer A, Bengsch B, Pircher H, Diepolder H, Gruner N, Jung M PLoS ONE, 2014;9(8):e105703. Species: Human Sample Type: Whole Cells Application: Flow Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection. Authors: Wang, Jia M, Shi, Lei, Ma, Cheng J, Ji, Xiao J, Ying, Ruo S, Wu, Xiao Y, Wang, Ke S, Li, Guangyu, Moorman, Jonathan, Yao, Zhi Q J Virol, 2013;87(8):4372-83. Species: Human Sample Type: Whole Cells Application: Flow Blockade of Tim-3 signaling restores the virus-specific CD8+ T-cell response in patients with chronic hepatitis B. Authors: Wu W, Shi Y, Li S, Zhang Y, Liu Y, Wu Y, Chen Z Eur. J. Immunol., 2012;42(5):1180-91. Species: Human Sample Type: Whole Cells Application: Flow Tim-3 negatively regulates IL-12 expression by monocytes in HCV infection. Authors: Zhang Y, Ma CJ, Wang JM, Ji XJ, Wu XY, Jia ZS, Moorman JP, Yao ZQ PLoS ONE, 2011;6(5):e19664. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human TIM‑3 Ser22-Arg200 Accession # Q8TDQ0.2
生物活性	Human
标记	Allophycocyanin
背景	TIM-3 (T cell Immunoglobulin and Mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4⁺ Th1 and CD8⁺ Tc1) but not on helper T cells (CD4⁺ Th2 and CD8⁺ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 6). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and proapoptotic signaling (7). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 6, 8), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (9).
运输条件	Blue Ice
存放说明	4℃
参考文献	Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362. Monney, L. et al. (2002) Nature 415:536. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102. Zhu, C. et al. (2005) Nat. Immunol. 6:1245. Koguchi, K. et al. (2006) J. Exp. Med. 203:1413. Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.

参考图片

Detection of TIM‑3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human TIM‑3 APC‑conjugated Monoclonal Antibody (Catalog # FAB2365A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.