Human HLA Class I PerCP MAb (Clone W6/32) (100 TESTS)
货号：
FAB7098C 基本售价：
5234.8 元 规格：
100 Tests
产品信息
概述| 货号 | FAB7098C |
| 别名 | A-10 alpha chain; FLJ26655; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-28 alpha chain; HLA class I histocompatibility antigen, A-9 alpha chain; HLAA; HLA-A; HLA-ABC; major histocompatibility complex, class I, A; MHC class I antigen A*1; MHC class I antigen A*11; MHC class I antigen A*80 |
全称 | Class I Human Leukocyte Antigen | | 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects the human major histocompatibility complex (MHC) class I, HLA-A, B, and C. Recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both the HLA molecule and beta 2-Microglobulin. |
| 使用方法 | Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 3105 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Membranes from human tonsillar lymphocytes
|
| 生物活性 | Human |
| 标记 | PerCP |
| 背景 | The MHC (Major Histocompatibility Complex) is a group of atleast 200 genes located on chromosome 6 in human. It contains multiple groupings, one of whichis called Class I that contains three distinct, but closely relatedmolecules. These three molecules areknown as MHC Class I-A, -B, and -C which, in the human, have been renamed HLA(Human Leukocyte Antigen)-A, -B, and -C. All are 44-46 kDa type I transmembrane glycoproteins that shareapproximately 85% amino acid sequence identity in their extracellulardomains. And all represent the alpha - component of a alpha - beta heterodimer that utilizes the 11-12 kDa transmembrane beta 2-microglobulin protein as a beta -component. These HLA heterodimers appear onall nucleated cells, and serve as a platform for the presentation ofcytoplasmic components (both self and foreign) to the alpha beta -TCR of cytotoxic CD8+ T cells. Unedited or mutated "self" components shouldbe ignored, while tumor or viral components should elicit a cytotoxic immuneresponse. This requires the continuousinternal "processing" or degradation of large proteins into 8-10 amino acidpeptides that are subsequently bound to a type A, B or C heterodimer and cycledto the plasma membrane. On the cellsurface, the B chain is most common while the C chain is least common. And with advancing age, both the A and Bchains decline in number. Not all A, Band C chains are "engaged"; while 90% of the HLA B chains are associated withantigen, only 30-70% of A and C chains are associated with processedantigens. And identical peptides can beperceived differently. For instance, anine amino acid peptide with O-linked (but not N-linked) glycosylation will notbe recognized by a CD8+ T cell that is specific for the naked nine amino acidpeptide. Finally, the A, B and C chains,if not the entire heterodimeric complex, are now known to act in-cis with LILRB2, generating anactivating complex on select cell types. The mouse MHC counterpart to the human HLAsystem is called H-2, and the two mouse genes that correspond to human HLA-Aand -B show 68% amino acid sequence identity over their entire lengths. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Dendritic Cell Co-stimulatory Molecules
- Mesenchymal Stem Cell Markers
- T Cell Antigen Recognition
|
参考图片 Detection of HLA Class I in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA Class I PerCP‑conjugated Monoclonal Antibody (Catalog # FAB7098C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins. |
