概述货号 | IC1420C |
别名 | AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin |
全称 | Actin, Alpha 2, Smooth Muscle, Aorta | 反应种属 | Human |
应用 | Intracellular Staining by Flow Cytometry |
目标/特异性 | Detects human alpha -Smooth Muscle Actin. |
使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells
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来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 | |
性能供应商 | R&D Systems |
Entrez Gene IDs | 59 (Human); 11475 (Mouse); 81633 (Rat) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide |
生物活性 | Human |
标记 | PerCP |
背景 | alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2). |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 | - Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
- Oishi, K. et al. (2002) J. Physiol. 540:139.
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参考图片 Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human alpha ‑Smooth Muscle Actin PerCP‑conjugated Monoclonal Antibody (Catalog # IC1420C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |