| 货号 | FAB7498R-100UG |
| 别名 | D6Bwg0781e; Img; LIG1; LIG-1; LRIG1 | 全称 | Leucine-rich Repeats and Immunoglobulin-like Domains 1 |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human LRIG1 in ELISAs.In direct ELISAs, less than 5%cross-reactivity with recombinant mouse LRIG1 and recombinant human LRIG3 isobserved. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 26018 (Human); 16206 (Mouse); 312574 (Rat) |
| 免疫原 | HEK293 human embryonic kidney cell line transfected with human LRIG1 Ala35-Ser779 Accession # Q96JA1 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 647 |
| 背景 | LRIG1 (leucine-rich repeats and Ig-like domains-1; also LIG-1) is an approximately 134-145 kDa glycoprotein that belongs to the LRIG gene family. It is widely expressed and appears on the surface of prostatic epithelium, endothelial cells, vascular and visceral smooth muscle, mammary epithelium, cardiac muscle, keratinocytes and neurons. LRIG1 is believed to negatively regulate the ErbB family of receptors. In particular, and in a ligand-independent manner, LRIG1 complexes with all four ErbBs, promoting their ubiquitination and decreasing their number. Alternatively, LRIG1 is suggested to bind to the ErbBs, preventing their dimerization and signal transduction. Mature human LRIG1 is a 1059 amino acid (aa) type I transmembrane protein. It contains a large 760 amino acid (aa) extracellular domain (ECD) (aa 35-794) plus a 278 aa cytoplasmic region. The ECD contains 17 LRRs (aa 35‑491) and three C2-type Ig-like domains (aa 495-780). These two domain types are each sufficient for EGFR binding. There are two potential alternative splice forms. One contains a 27 aa insertion after Gly874, while another shows a 24 aa insertion after Lys387 coupled to a Gln substitution for aa 644-691. The LRIG1 ECD undergoes proteolysis, generating 100-110 and 55-60 kDa soluble fragments. Over aa 35‑779, human LRIG1 shares 90% aa sequence identity with mouse LRIG1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|