| 货号 | FAB4694N-100UG |
| 别名 | CORD10; RP35; Sema B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4A; SEMA4A; SEMAB; Semaphorin 4A; semaphorin-4A; semaphorin-B; SEMB; SEMBFLJ12287 |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human Semaphorin 4A in direct ELISAs. In direct ELISAs, 50%-100% cross-reactivity withrecombinant human (rh) Semaphorin 4C and rhSemaphorin 4G is observed, and lessthan 5% cross-reactivity with rhSemaphorin 4B, 4D, and recombinant mouseSemaphorin 4A is observed. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 64218 (Human); 20351 (Mouse); 310630 (Rat) |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4A Gly32-His683 Accession # Q9H3S1 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 700 |
| 背景 | Semaphorin 4A (Sema4A, previously semB) is a Class 4 transmembrane Semaphorin with activity in the immune and nervous systems (1). The 761 amino acid (aa) human Sema4A precursor contains a 32 aa signal sequence, a 651 aa extracellular domain (ECD) containing sema, PSI and C2-type immunoglobulin domains, a 21 aa transmembrane domain, and a 57 aa cytoplasmic domain with two Ser/Thr phosphorylation sites (2). Human Sema4A ECD shares 87%, 87%, 86% and 85% aa identity with mouse, rat, bovine and canine Sema4A, respectively, and shares 32-37% aa identity with other human Sema4 family members. Of six reported splice variants with 723, 629, 370, 321, 236 and 220 aa, five lack the N-terminus and/or portions of the sema domain, and three lack the transmembrane and cytoplasmic domains in the C-terminus (3). Sema4A was first described as a molecule that enhances T cell activation and interacts with TIM-2 (T cell immunoglobulin and mucin domain-2 (4). Mice with targeted disruption of Sema4A show defects in dendritic cell-mediated T cell priming and Th1 responses (5). Roles for Sema4A have also been identified in the brain, the endothelium and the eye. It mediates hippocampal neuron growth cone collapse in vitro through interaction of the sema domain with Plexin-B1 (6). Interaction of Sema4A with endothelial cell Plexin-D1 causes opposition to the angiogenic, proliferative, chemotactic and integrin-mediated adhesive actions of VEGF (7). The retina of Sema4A-/- mice shows severe degeneration, and mutations of Sema4A are associated with retinitis pigmentosa and cone rod dystrophy in humans (8, 9). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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