| 货号 | FAB4204N-100UG |
| 别名 | 2310008D05Rik; endothelial cell adhesion molecule; endothelial cell-selective adhesion molecule; ESAM; HUEL (C4orf1)-interacting protein; LP4791 protein; W117m | 全称 | Endothelial Cell Adhesion Molecule |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human ESAM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ESAM is observed. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 90952 (Human); 69524 (Mouse) |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human ESAM Gln30-Ala247 Accession # Q96AP7 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 700 |
| 背景 | Endothelial cell-selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules (1, 2). Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion (1). In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI-1 (3). The extracellular region of human ESAM shows 90%, 74%, 69%, and 67% aa identity with monkey, canine, mouse, and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets, and megakaryocytes and can be found associated with cell-to-cell junctions. Whether ESAM is restricted to a particular junctional type is not clear (1, 2). ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF-2 (4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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