| 货号 | IC2701U-100UG |
| 别名 | CCL3/CCL4 | 全称 | CCL3/MIP-1 alpha and CCL4/MIP-1 beta Cross-reactive |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human CCL3/MIP-1 alpha in Western blots. Indirect ELISAs, 100% cross-reactivity with recombinant human (rh) CCL4/MIP-1 beta isobserved. Does not cross-react with rhCCL1, 2, 5, 7, 8, 11, 13, 14, 15, 16, 17,18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 2, 3, 5, 6, 7,CCL9/10MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | E. coli-derived recombinant human CCL3/MIP-1 alpha Ala27-Ala92 Accession # P10147 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 350 |
| 背景 | The macrophage inflammatory proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP-1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP-1 alpha. Mature MIP-1 alpha and MIP-1 beta in both human and mouse share approximately 70% homology at the amino acid level. The MIP‑1 proteins are members of the beta (C-C) subfamily of chemokines. Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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