| 货号 | FAB7824G-100UG |
| 别名 | DCSTAMP; DC-STAMP; dendritic cell-specific transmembrane protein; FIND; IL-4-induced Protein; TM7SF4; transmembrane 7 superfamily member 4 | 全称 | Dendritic Cell-specific Transmembrane Protein |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human DC-STAMP in direct ELISAs.In direct ELISAs, no cross-reactivitywith recombinant mouse DC-STAMP is observed. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 81501 (Human); 75766 (Mouse); 299889 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human DC-STAMP Asp314-Thr376 Accession # Q9H295 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 488 |
| 背景 | DC-STAMP, also known as TM7SF4, is an approximately 50 kDa glycoprotein with seven transmembrane segments. It is expressed on the surface of dendritic cells and monocytes as well as on osteoclasts and their progenitors. DC-STAMP binds CTGF/CCN2 and cooperates with TRANCE/RANK L for inducing osteoclast differentiation and fusion into multinucleated cells. It contains a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and associates with FC gamma RIII/CD16. Within aa 314-376, human DC-STAMP shares approximately 75% aa sequence identity with mouse and rat DC-STAMP. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of DC-STAMP in Human Denditic Cells by Flow Cytometry. Mature human monocyte-derived dendritic cells treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mLRecombinant Human GM-CSF (Catalog # 215-GM) for 6 days then treated with 20 ng/mL LPS, 20 ng/mL Recombinant Human TNF-alpha (Catalog # 210-TA), and 20 ng/ml Recombinant Human IL-1 beta (Catalog # 201-LB) for 24 hours were stained with Mouse Anti-Human DC-STAMP Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7824G, filled histogram) or Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins. |
