Human OSM R beta Allophycocyanin MAb (Clone 469221) (100 TESTS)【科瑞奥博】

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Human OSM R beta Allophycocyanin MAb (Clone 469221) (100 TESTS)

货号： FAB4389A 基本售价： 5086.1 元规格： 100 Tests

产品信息

概述
货号	FAB4389A
别名	OSMR; OSMRB
全称	Oncostatin M Receptor beta
反应种属	Human
应用	Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human OSM R beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse OSM R beta is observed.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	9180 (Human); 18414 (Mouse); 310132 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Prerequisites for Functional Interleukin 31 Signaling and Its Feedback Regulation by Suppressor of Cytokine Signaling 3 (SOCS3). Authors: Maier E, Mittermeir M, Ess S, Neuper T, Schmiedlechner A, Duschl A, Horejs-Hoeck J J Biol Chem, 2015;290(41):24747-59. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human OSM R beta Glu28-Ser739 Accession # Q99650
生物活性	Human
标记	Allophycocyanin
背景	OSM R beta is a 150‑180 kDa member of the IL-6 receptor family. It associates with gp130 to form the type II OSM receptor that is responsive to OSM. The gp130 subunit is shared by other IL-6 family cytokine receptors (1, 2, 3, 4), and OSM R beta associates with gp130-like receptor (GPL) to form a receptor complex responsive to IL-31 (5, 6). The human OSM R beta cDNA encodes a 979 amino acid (aa) precursor that includes a 27 aa signal sequence, a 712 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 218 aa cytoplasmic domain. The ECD contains one partial and one complete hematopoietin domain, an Ig-like domain, and three fibronectin type-III domains. The cytoplasmic domain contains box1, 2, and 3 motifs (7). Within the ECD, human OSM R beta shares 55%, 58%, 61%, and 72% aa sequence identity with mouse, rat, bovine, and canine OSM R beta, respectively. It also shares 31% aa sequence identity with human LIF R, but less than 20% aa sequence identity with human CNTF R alpha, G-CSF R, IL-6 R, IL-11 R alpha, and TCCR. OSM R beta does not bind cytokines directly, but increases the affinity of gp130 for OSM, and GPL for IL-31 (7, 8). OSM R beta, gp130, and GPL each initiate signaling events following ligand stimulation (9, 10). Jak/STAT and MAPK pathways are activated by OSM R beta -containing receptors (9, 11, 12, 13), including STAT5b and SHC which are not activated by other IL-6 family receptors (10, 13). In mice, the loss of OSM R beta expression blocks erythroid progenitor development in bone marrow, and dramatically reduces the number of circulating platelets and erythrocytes (14). The type II OSM receptor is the only IL-6 family receptor that promotes osteoblast differentiation in calvaria cell cultures (15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Chen, S.-H. and E.N. Benveniste (2004) Cytokine Growth Factor Rev. 15:379. Heinrich, P.C. et al. (2003) Biochem. J. 374:1. Tanaka, M. and A. Miyajima (2003) Rev. Physiol. Biochem. Pharmacol. 149:39. Gearing, D.P. et al. (1992) Science 255:1434. Dillon, S.R. et al. (2004) Nat. Immunol. 5:752. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850. Mosley, B. et al. (1996) J. Biol. Chem. 271:32635. Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291. Dreuw, A. et al. (2004) J. Biol. Chem. 279:36112. Wang, Y. et al. (2000) J. Biol. Chem. 275:25273. Hermanns, H.M. et al. (2000) J. Biol. Chem. 275:40742. Kuropatwinski, K.K. et al. (1997) J. Biol. Chem. 272:15135. Auguste, P. et al. (1997) J. Biol. Chem. 272:15760. Tanaka, M. et al. (2003) Blood 102:3154. Malaval, L. et al. (2005) J. Cell. Physiol. 204:585.

参考图片

Detection of OSM R beta in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human OSM R beta APC-conjugated Monoclonal Antibody (Catalog # FAB4389A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.