| 货号 | IC317N |
| 别名 | CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A | 全称 | Interleukin 17 |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects human IL-17 in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17 is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human IL-17 Ile20-Ala155 Accession # Q16552 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 700 |
| 背景 | Interleukin 17, also known as IL-17A and CTLA-8, is a 20-22 kDa, secreted pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield the 132 aa residue mature IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 is also known to form a heterodimer with 20-24 kDa IL-17F. At the amino acid level, hIL-17 shows 62% aa sequence identity with mouse IL-17. The receptor for the IL-17A homodimer and IL-17A:F heterodimer is reported to be a combination of IL-17 RA and IL-17 RC, with a possible contribution by IL-17 RD. The expression of IL-17 is widespread, and found associated with mast cells, LTi cells, B cells, gamma δ T cells, CD4+ Th17 cells, iNKT cells, neutrophils, intestinal Paneth cells, Type I ILCs and CD8+Tc17 cells. IL-17 exhibits multiple biological activities on a variety of cells, including: the induction of IL-6 and IL-8 production by fibroblasts, the enhancement of surface expression of ICAM-1 on fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation, the preservation of intestinal mucosal integrity via claudin synthesis, and the induction of antimicrobial peptides by epithelium. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IL‑17/IL‑17A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Mouse Anti-Human IL‑17/IL‑17A Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # IC317N) and Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # IC0041N). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |
