| 货号 | FAB4286A |
| 别名 | BG37; BG37hBG37; G protein-coupled bile acid receptor 1; GPBA; GPBAR1; GPCR19; GPR131; G-protein coupled bile acid receptor 1; G-protein coupled bile acid receptor BG37; G-protein coupled receptor GPCR19; M-BAR; M-BARhGPCR19; membrane bile acid receptor; Membrane-type receptor for bile acids; MGC40597; TGR5GPCR | 全称 | Bile Acid Receptor TGR5 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Stains human TGR5/GPBAR1 transfectants but not irrelevant transfectants in flow cytometry. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 151306 (Human); 227289 (Mouse); 338443 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0 mouse myeloma cell line transfected with human TGR5/GPBAR1 Met1-Asn330 Accession # Q8TDU6 |
| 生物活性 | Human |
| 标记 | Allophycocyanin |
| 背景 | TGR5, also known as GPR131, GPBAR1, M-BAR, and BG37, is a 46-48 kDa member of the GPCR1 family of molecules. It is a 330 amino acid (aa) 7-transmembrane glycoprotein that is found on a wide variety of cell types. These include monocytes, macrophages, Kupffer cells, skeletal muscle and pancreatic acinar cells, autonomic enteric and sensory DRG neurons, liver sinusoidal endothelial cells, intestinal L and EC (enterochromaffin) cells, gallbladder epithelium and cholangiocytes (bile duct epithelium). Notably, TGR5 expression exhibits polarity with respect to plasma membrane location, and it may appear either on the cell surface or in intracellualr membrane compartments such as the ER, nucleus or multivesicular bodies. Initially considered to be coupled to G alpha s with cAMP production, it is now known to also interact with G alpha i, activating the ERK signaling pathway. Bile acids generated from cholesterol in hepatocytes are a known ligand for TGR5. Aside from their role in fatty acid absorption, BA ligation of macrophage/Kupffer cell TGR5 results in an anti-inflammatory environment. Over the aa that constitute the TGR5 extracellular domain, human and mouse share 73% aa sequence identity; over the entire ORF, human and mouse share 83% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of TGR5/GPBAR1 in HEK293 Human Cell Line by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TGR5/GPBAR1 and eGFP was stained with either (A) Mouse Anti-Human TGR5/GPBAR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB4286A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins. |
