| 货号 | FAB3649A |
| 别名 | AGR16; EDG5; EDG-5; endothelial differentiation G-protein coupled receptor 5; endothelial differentiation, sphingolipid G-protein-coupled receptor, 5; GPCR13; GPCR18; H218; LPB2; S1P receptor 2; S1P receptor EDG5; S1P receptor Edg-5; S1P2; S1PR2; sphingosine 1-phosphate receptor 2; sphingosine 1-phosphate receptor Edg-5; sphingosine-1-phosphate receptor 2 | 全称 | Endothelial Differentiation Gene 5 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human S1P2/EDG-5. Stains human S1P2/EDG-5 transfectants but not irrelevant transfectants. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9294 (Human); 14739 (Mouse); 29415 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0 mouse myeloma cell line transfected with human S1P2/EDG-5 Met1-Val353 Accession # AAC98919 |
| 生物活性 | Human |
| 标记 | Allophycocyanin |
| 背景 | S1P2 (sphingosine 1-phosphate receptor-2), also known as EDG-5 (endothelial differentiation, G-protein coupled receptor-5), is a353 amino acid (aa) seven-transmembrane receptor putative glycoproteinthat binds the lysolipid phosphoric acid mediator, sphingosine1-phosphate. Extracellular portions of human S1P2 show 82.5% aaidentity with mouse S1P2 and 92% aa identity with human EDG-3, themost closely related family member. Both are expressed predominantly inthe lung, heart, kidney, liver, spleen, thymus, testis and brain,mediating both mitogenic and anti-apoptotic effects. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of S1P2/EDG-5 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human S1P2/EDG-5 APC‑conjugated Monoclonal Antibody (Catalog # FAB3649A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins. | |
Detection of S1P2/EDG-5 in HEK293 Human Cell Line Transfected with Human S1P2/EDG-5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human S1P2/EDG-5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human S1P2/EDG-5 APC‑conjugated Monoclonal Antibody (Catalog # FAB3649A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins. |
