Human Granzyme B PerCP MAb (Clone 351927) (100 TESTS)【科瑞奥博】

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Human Granzyme B PerCP MAb (Clone 351927) (100 TESTS)

货号： IC2906C 基本售价： 5086.1 元规格： 100 Tests

产品信息

概述
货号	IC2906C
别名	CSPB; CTLA-1; CTSGL1; Fragmentin-2; Granzyme-2; GRB; GrzB; GZMB; HLP; SECT
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human Granzyme B in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Granzyme A, rhGranzyme H, recombinant mouse (rm) Granzyme B, rmGranzyme C, rmGranzyme D, or rmGranzyme G.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3002 (Human); 14939 (Mouse)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human Granzyme B Gly19-Tyr247 Accession # P10144
生物活性	Human
标记	PerCP
背景	Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Human Granzyme B is synthesized as a precursor (247 residues) with a signal peptide (residues 1-18), a pro peptide (residues 19-20), and a mature chain (residues 21-247) (4-6). The recombinant human (rh) Granzyme B consisting of residues 19-247 was expressed and purified. After being activated by active cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3).
运输条件	Blue Ice
存放说明	4℃
参考文献	Kam, C-M. et al. (2000) Biochim. Biophys. Acta 1477:307. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al., eds., pp. 1549 - 1552. Schmid, J. and C. Weissman (1987) J. Immunol. 139:250. Caputo, A. et al. (1988) J. Biol. Chem. 263:6363. Trapani, J.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6924.

参考图片

Detection of Granzyme B in NK‑92 Human Cell Line by Flow Cytometry. NK‑92 human natural killer lymphoma cell line was stained with Mouse Anti-Human Granzyme B PerCP‑conjugated Monoclonal Antibody (Catalog # IC2906C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.