Human Vimentin Allophycocyanin MAb (Clone 280618) (100 TESTS)【科瑞奥博】

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Human Vimentin Allophycocyanin MAb (Clone 280618) (100 TESTS)

货号： IC2105A 基本售价： 5086.1 元规格： 100 Tests

产品信息

概述
货号	IC2105A
别名	FLJ36605; VIM; vimentin
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human Vimentin in Western blots.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7431 (Human); 22352 (Mouse); 81818 (Rat)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human Vimentin Ser2-Glu466 Accession # P08670
生物活性	Human
标记	Allophycocyanin
背景	Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1-3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine, and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10-12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9-11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Omary, M.B. et al. (2006) Trends Biochem. Sci. 31:383. Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050. Ferrari, S. et al. (1986) Mol. Cell. Biol. 6:3614. Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206. Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919. Li, Q-F. et al. (2006) J. Biol. Chem. 281:34716. Esue, O. et al. (2006) J. Biol. Chem. 281:30393. Styers, M.L. et al. (2005) Traffic 6:359. McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109. Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156. Ivaska, J. et al. (2005) EMBO J. 24:3834. Byun, Y. et al. (2001) Cell Death Differ. 8:443. Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59. Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625. Garg, A. et al. (2006) J. Immunol. 177:6192.

参考图片

Detection of Vimentin in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Rat Anti-Human Vimentin APC‑conjugated Monoclonal Antibody (Catalog # IC2105A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.