货号 | IC2167A-100 |
别名 | D12S1644; EC 2.4.1.227; EC 2.7.7.6; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin4-induced; STAT6B; STAT6C; transcription factor IL-4 STAT | 全称 | Signal Transducer and Activator of Transcription 6 |
反应种属 | Human |
应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
目标/特异性 | Detects human STAT6 in Western blots. |
使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 6778 (Human); 20852 (Mouse); 362896 (Rat) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant human STAT6 Asn341-Gly640 Accession # P42226.1 |
生物活性 | Human |
标记 | Allophycocyanin |
背景 | STAT family proteins mediate cytokine signaling by acting as signaltransducers in the cytoplasm and transcription activators in thenucleus. STAT6 is important for the development of Th2 cells andregulation of gene expression induced by IL-4 and IL-13. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of STAT6 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human STAT6 APC‑conjugated Monoclonal Antibody (Catalog # IC2167A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |