Human GITR/TNFRSF18 Allophycocyanin MAb (Clone 110416) (100 TESTS)【科瑞奥博】

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Human GITR/TNFRSF18 Allophycocyanin MAb (Clone 110416) (100 TESTS)

货号： FAB689A-100 基本售价： 5086.1 元规格： 100 Tests

产品信息

概述
货号	FAB689A-100
别名	Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18
全称	Glucocorticoid Induced TNF Receptor Family Related Gene
反应种属	Human
应用	Flow Cytometry
目标/特异性	Detects human GITR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4‑1BB, recombinant mouse (rm) 4‑1BB, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFAS, rmGITR, rhHVEM, rhLymphotoxin R beta, rmLymphotoxin R beta, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Expression of human GITRL on myeloid dendritic cells enhances their immunostimulatory function but does not abrogate the suppressive effect of CD4+CD25+ regulatory T cells. Authors: Tuyaerts S, Van Meirvenne S, Bonehill A, Heirman C, Corthals J, Waldmann H, Breckpot K, Thielemans K, Aerts JL J. Leukoc. Biol., 2007;82(1):93-105. Species: Human Sample Type: Whole Cells Application: Flow CD4+CD25+ regulatory T-cell frequency in HER-2/neu (HER)-positive and HER-negative advanced-stage breast cancer patients. Authors: Perez SA, Karamouzis MV, Skarlos DV, Ardavanis A, Sotiriadou NN, Iliopoulou EG, Salagianni ML, Orphanos G, Baxevanis CN, Rigatos G, Papamichail M Clin. Cancer Res., 2007;13(9):2714-21. Species: Human Sample Type: Blood Application: Flow Increase of CD4+ CD25+ regulatory T-cells in the liver of patients with hepatocellular carcinoma. Authors: Yang XH, Yamagiwa S, Ichida T, Matsuda Y, Sugahara S, Watanabe H, Sato Y, Abo T, Horwitz DA, Aoyagi Y J. Hepatol., 2006;45(2):254-62. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18 Gln26-Glu161 (Thr43Ala) Accession # Q9Y5U5
生物活性	Human
标记	Allophycocyanin
背景	GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up‑regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.
运输条件	Blue Ice
存放说明	4℃
参考文献	Nocentini, G.et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216. Kwon, B.et al. (1999) J. Biol. Chem. 274:6056. Gurney, A.L.et al. (1999) Current Biology 9:215. Kwon, B.et al. (1999) Current Opinion in Immunology 11:340.

参考图片

Detection of GITR/TNFRSF18 in Human Blood CD4⁺ Lymphocytes by Flow Cytometry. Human peripheral blood CD4⁺ lymphocytes treated with PHA for 5 days were stained with Mouse Anti-Human GITR/TNFRSF18 APC‑conjugated Monoclonal Antibody (Catalog # FAB689A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.