Human APRIL/TNFSF13 Allophycocyanin Affinity Purified PAb (100 TESTS)
货号：
IC884A 基本售价：
4947.3 元 规格：
100 Tests
产品信息
概述| 货号 | IC884A |
| 别名 | A proliferation-inducing ligand; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2 |
全称 | A Proliferation-inducing Ligand | | 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells)
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| 目标/特异性 | Detects human APRIL/TNFSF13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Fas Ligand, rhTNF-alpha, rhTRAIL, and rhTRANCE is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 8741 (Human); 69583 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human APRIL/TNFSF13 |
| 生物活性 | Human |
| 标记 | Allophycocyanin |
| 背景 | APRIL (A Proliferation-Inducing Ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily (1). APRIL is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule (2, 3). Secreted APRIL consists almost entirely of a single TNF homology domain (2, 3). Little or no transmembrane APRIL is expressed on the cell surface (3). Alternate splicing generates isoforms with short deletions at the N- or C-terminus (4). Human APRIL shares 85% aa sequence identity with mouse and rat APRIL. Among TNF superfamily ligands, BAFF shows the greatest sequence homology with APRIL, and the two proteins exhibit overlapping biological activities. APRIL promotes cellular proliferation and protects from apoptosis in normal and transformed cells (3, 5-7). It is present in elevated amounts in a wide variety of cancers primarily due to expression by tumor-infiltrating neutrophils (4, 5, 7-9). Both APRIL and BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, and BAFF additionally functions through BAFF R (6, 10, 11). A stretch of basic amino acids at the N-terminus of APRIL is required for its interaction with Heparan Sulfate Proteoglycans (HSPGs) (12, 13). Binding to HSPGs is independent of APRIL’s binding to TACI and BCMA (12, 13). Interaction with HSPGs serves to concentrate APRIL on the surface of cells, thereby favoring TACI- or BCMA-mediated effects (8, 9, 13). APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders (14). A bioactive protein known as TWE-PRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL (15). TWE-PRIL is expressed in monocytes and activated T cells, and in contrast to APRIL, it is presented on the cell surface (15). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Planelles, L. et al. (2008) Curr. Mol. Med. 8:829.
- Hahne, M. et al. (1998) J. Exp. Med. 188:1185.
- Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945.
- Kelly, K. et al. (2000) Cancer Res. 60:1021.
- Roth, W. et al. (2001) Cell Death Differ. 8:403.
- Yu, G. et al. (2000) Nat. Immunol. 1:252.
- He, B. et al. (2004) J. Immunol. 172:3268.
- Schwaller, J. et al. (2007) Blood 109:331.
- Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.
- Day, E.S. et al. (2005) Biochemistry 44:1919.
- Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
- Hendriks, J. et al. (2005) Cell Death Differ. 12:637.
- Ingold, K. et al. (2005) J. Exp. Med. 201:1375.
- Roschke, V. et al. (2002) J. Immunol. 169:4314.
- Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.
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参考图片Detection of APRIL/TNFSF13 in Th2-stimulated PBMCs by Flow Cytometry. Th2-stimulated peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human APRIL/TNFSF13 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC884A) and Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # IC108A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
