| 货号 | FAB6136A |
| 别名 | ectonucleotide pyrophosphatase/phosphodiesterase 1; E-NPP 1; Ly-41 Antigen; M6S1; M6S1NPP1; Membrane component chromosome 6 surface marker 1; membrane component, chromosome 6, surface marker 1; NPP1; NPPS; NPPSalkaline phosphodiesterase 1; PC1; PC-1; PCA1; PCA1ARHR2; PDNP1; PDNP1ectonucleotide pyrophosphatase/phosphodiesterase family member 1; Phosphodiesterase I/nucleotide pyrophosphatase 1; plasma-cell membrane glycoprotein 1; Plasma-cell membrane glycoprotein PC-1 | 全称 | Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human ENPP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human ENPP-2 and recombinant mouse ENPP-2 is observed after removal of cross-reactivity with human ENPP-2. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5167 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human ENPP-2-1-2 Asp49-Trp144 (ENPP-2), Val191-Leu591 (ENPP-1), Asn532-Ile863 (ENPP-2). Cross-reactivity with human ENPP-2 was removed from the final product. Accession # Q13822 (ENPP-2) P22413 (ENPP-1) |
| 生物活性 | Human |
| 标记 | Allophycocyanin |
| 背景 | Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP-1) is a transmembrane glycoprotein that hydrolyzes nucleotides and nucleotide derivatives with the formation of nucleotide-5’-monophosphates. It is inserted into the plasma membrane by an N‑terminal transmembrane domain. Human ENPP-1 has a small N-terminal cytoplasmic domain and a large C-terminal region containing two somatomedin B-like domains, a catalytic domain and a nuclease-like domain in the extracellular space (1). Defects in the ENPP-1 gene cause arterial calcification and bone mineralization abnormalities (2). ENPP-1 polymorphism or overexpression is also associated with obesity, type II diabetes and insulin resistance, which makes modulation of ENPP-1 activity one of the targets to treat insulin resistance and related diseases (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of ENPP‑1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human ENPP‑1 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6136A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins. |
