概述| 货号 | FAB1264N |
| 别名 | alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA |
全称 | Platelet-derived Growth Factor Receptor alpha | | 反应种属 | Human |
| 应用 | Flow Cytometry(5 µL/106cells)
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| 目标/特异性 | Detects human PDGF R alpha. Recognizes the PDGF receptor alpha -subunit; it does not recognize the PDGF receptor beta -subunit. Binds to the PDGF receptor alpha -subunit of primate species (human, monkey, baboon) and dog. Does not recognize rat or mouse receptors and its ability to bind to receptors from other species has not been tested. |
| 使用方法 | Flow Cytometry: 5 µL/106cells
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 5156 (Human); 18595 (Mouse) |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | Human osteosarcoma cell membrane extracts |
| 生物活性 | Human |
| 标记 | Alexa Fluor 700 |
| 背景 | PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.
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参考图片Detection of PDGF R alpha in U‑118‑MG Human Cell Line by Flow Cytometry. U‑118‑MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human PDGF R alpha Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB1264N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins. |
