Human IDO Allophycocyanin Monoclonal Ab (Clone 700838) (100 TESTS)【科瑞奥博】

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Human IDO Allophycocyanin Monoclonal Ab (Clone 700838) (100 TESTS)

货号： IC6030A 基本售价： 4947.3 元规格： 100 Tests

产品信息

概述
货号	IC6030A
别名	EC 1.13.11.52; IDO1; IDOIDO-1; INDO; INDOindole 2,3-dioxygenase; indoleamine 2,3-dioxygenase 1; indoleamine-pyrrole 2,3 dioxygenase; Indoleamine-pyrrole 2,3-dioxygenase
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human Indoleamine 2,3-dioxygenase/IDO in direct ELISA.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3620 (Human); 15930 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Monocyte Differentiation towards Protumor Activity Does Not Correlate with M1 or M2 Phenotypes Authors: G Karina Chimal-Ram J Immunol Res, 2016;2016(0):6031486. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human Indoleamine 2,3-dioxygenase/IDO Ala2-Gly403 Accession # P14902
生物活性	Human
标记	Allophycocyanin
背景	Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine (1). This degradation is the first and rate-limiting step of the L-kynurenine pathway (2). IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN-gamma, IFN-alpha, IFN-beta, and IL‑10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments (3). Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia (3-5).
运输条件	Blue Ice
存放说明	4℃
参考文献	Lewis-Ballester, A. et al. (2009) Proc. Natl. Acad. Sci. USA. 106:17371. Costantino, G. (2009) Expert Opin. Ther. Targets 13:247. Xu, H. et al. (2008) Immunol. Lett.121:1. Lob, S. et al. (2009) Nat. Rev. Cancer 9:445. Curti, A. et al. (2009) Blood 113:2394.

参考图片

Detection of Indoleamine 2,3‑dioxygenase/IDO in Human MDSCs by Flow Cytometry. Human PBMC-derived myeloid-derived suppressor cells (MDSCs) treated with 10 ng/mL Recombinant Human IL‑6 (Catalog # 206-IL) and 10 ng/mL Recombinant Human GM‑CSF (Catalog # 215-GM) for 7 days were stained with Mouse Anti-Human Siglec‑3/CD33 PE‑conjugated Monoclonal Antibody (Catalog # FAB1137P) and either (A) Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO APC‑conjugated Monoclonal Antibody (Catalog # IC6030A) or (B) Mouse IgG₁ Allophycocyanin Isotype Control (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.