Human Cadherin-11 PerCP MAb (Clone 667039) (100 TESTS)【科瑞奥博】

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Human Cadherin-11 PerCP MAb (Clone 667039) (100 TESTS)

货号： FAB17901C 基本售价： 4947.3 元规格： 100 Tests

产品信息

概述
货号	FAB17901C
别名	CAD11OSF-4; cadherin 11, type 2, OB-cadherin (osteoblast); cadherin-11; CDH11; CDHOB; OB; OB-Cadherin; Osteoblast cadherin
反应种属	Human
应用	Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human Cadherin-11 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Cadherin-4, -6, -8, -12, -13, -17, rhE-Cadherin, rhN-Cadherin, rhP-Cadherin, or rhVE-Cadherin is observed.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1009 (Human); 12552 (Mouse)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human Cadherin-11 Phe23-Thr617 Accession # AAA35622
生物活性	Human
标记	PerCP
背景	Cadherin-11, also known as OB-Cadherin, is a 120 kDa member of the classical Cadherin family of calcium-dependent homophilic adhesion proteins. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1). Cadherin-11 is expressed in embryonic mesodermal tissues and contributes to the morphogenesis of the nervous and skeletal systems (2-4). It is expressed on osteoblasts in the adult where it promotes the differentiation of both osteoblasts and chondrocytes (5). Cadherin-11 is up‑regulated on breast cancer and prostate cancer cells which preferentially metastasize to bone (6, 7). It facilitates this metastasis via homophilic adhesion to bone marrow stroma and osteoblast-expressed Cadherin-11 (6‑8). In the synovium, Cadherin‑11 supports adhesion between synoviocytes but promotes cell invasion in synovitis and rheumatoid arthritis (9, 10). Its up‑regulation in the vasculature following injury contributes to intimal hyperplasia by inducing smooth muscle cell migration and proliferation (11). In the nervous system, Cadherin-11 interacts with FGF R1 to promote neurite extension from spinal cord explants (12). Mature human Cadherin‑11 consists of a 564 amino acid (aa) extracellular domain (ECD) with five tandem Cadherin repeats, a 23 aa transmembrane segment, and a 156 aa cytoplasmic domain (13, 14). Within the ECD, human Cadherin-11 shares 97% and 98% aa sequence identity with mouse and rat Cadherin‑11, respectively. An 80 kDa portion of the Cadherin‑11 ECD can be shed by proteolytic cleavage, and this fragment competes with the full length molecule for cell adhesion (3, 15). Alternate splicing of human Cadherin-11 generates an 85 kDa isoform with substituted transmembrane and cytoplasmic regions (14, 15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237. Kimura, Y. et al. (1995) Dev. Biol. 169:347. McCusker, C. et al. (2009) Mol. Biol. Cell 20:78. Clendenon, S.G. et al. (2009) Dev. Dyn. 238:1909. Kii, I. et al. (2004) J. Bone Mineral Res. 19:1840. Tamura, D. et al. (2008) Int. J. Oncol. 33:17. Chu, K. et al. (2008) Mol. Cancer Res. 6:1259. Huang, C.-F. et al. (2010) Cancer Res. 70:4580. Valencia, X. et al. (2004) J. Exp. Med. 200:1673. Kiener, H.P. et al. (2009) Arthritis Rheum. 60:1305. Monahan, T.S. et al. (2007) J. Vasc. Surg. 45:581. Boscher, C. and R.-M. Mege (2008) Cell. Signal. 20:1061. Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15. Okazaki, M. et al. (1994) J. Biol. Chem. 269:12092. Kawaguchi, J. et al. (1999) J. Bone Mineral Res. 14:764.

参考图片

Detection of Cadherin‑11 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Cadherin-11 PerCP-conjugated Monoclonal Antibody (Catalog # FAB17901C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). Cells were stained in a buffer containing Ca²⁺ and Mg²⁺. View our protocol for Staining Membrane-associated Proteins.