| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Stem cell-like side populations in esophageal cancer: a source of chemotherapy resistance and metastases.
Authors: Zhao Y, Bao Q, Schwarz B, Zhao L, Mysliwietz J, Ellwart J, Renner A, Hirner H, Niess H, Camaj P, Angele M, Gros S, Izbicki J, Jauch K, Nelson P, Bruns C
Stem Cells Dev, 2014;23(2):180-92.
Species: Human
Sample Type: whole cells
Application: Flow
MDR1 expression identifies human melanoma stem cells.
Authors: Keshet GI, Goldstein I, Itzhaki O, Cesarkas K, Shenhav L, Yakirevitch A, Treves AJ, Schachter J, Amariglio N, Rechavi G
Biochem. Biophys. Res. Commun., 2008;368(4):930-6.
Species: Human
Sample Type: Whole Cells
Application: Flow
Interaction with the 5D3 monoclonal antibody is regulated by intramolecular rearrangements but not by covalent dimer formation of the human ABCG2 multidrug transporter.
Authors: Ozvegy-Laczka C, Laczko R, Hegedus C, Litman T, Varady G, Goda K, Hegedus T, Dokholyan NV, Sorrentino BP, Varadi A, Sarkadi B
J. Biol. Chem., 2008;283(38):26059-70.
Species: Human
Sample Type: Whole Cells
Application: Flow
C421 allele-specific ABCG2 gene amplification confers resistance to the antitumor triazoloacridone C-1305 in human lung cancer cells.
Authors: Bram EE, Ifergan I, Grimberg M, Lemke K, Skladanowski A, Assaraf YG
Biochem. Pharmacol., 2007;74(1):41-53.
Species: Human
Sample Type: Whole Cells
Application: Flow |
| 背景 | Hematopoietic stem cells are known to express a membrane transporter molecule, known as P-glycoprotein (Pgp), that is encoded by the multidrug resistance gene 1 (MDR1) (1, 2). Expression of Pgp appears to confer a proliferative advantage to stem cells through its anti-apoptotic effects (3, 4). An additional transporter molecule known as ABCG2 (ATP-binding cassette gene 2) or Bcrp1 (Breast cancer resistance protein 1), first identified in a breast cancer cell line (5), is expressed on stem cells (6). ABCG2 belongs to a family of molecules that span the cell membrane six times and can exist as either homo or hetero dimers linked by a short intracellular flexible linker region that plays an important role in the efflux of a wide range of substrates (7, 8). Although these transporter molecules have initially been thought to play a role in drug resistance, they have been found to have utility in better characterizing primitive stem cells. For example, the “side-population” of hematopoietic stem cells, characterized by their inability to retain high levels of the intracellular staining dyes Hoechst 33342 and Rhodamine 123, has been found to express high levels of ABCG2. Of interest is the observation that ABCG2 function has been linked to the efflux of the Hoechst dye (6). Furthermore, there is now evidence that this monoclonal can be used as a cell surface marker to identify hematopoietic stem cells within the bone marrow fraction of lineage negative cells (6). The expression of ABCG2 appears greatest on CD34- cells and is downregulated with the acquisition of CD34 on the cell surface (6). |
| 参考文献 | - Chaudhary, P.M. and I.B. Roninson (1991) Cell 66:85.
- Sorrentino, B.P. et al. (1995) Blood 86:491.
- Pallis, M. and N. Russell (2000) Blood 95:2897.
- Johnstone, R.W. et al. (1999) Blood 93:1075.
- Doyle, L.A. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15665.
- Zhou, S. et al. (2001) Nat. Medicine 7:1028.
- Hrycyna, C.A. et al. (1998) Biochem. 37:13660.
- Bunting, K.D. (2002) Stem Cells 20:11.
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