| 货号 | IC4019A |
| 别名 | interferon regulatory factor 3; IRF-3 | 全称 | Interferon Regulatory Factor 3 |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human IRF3 in Western blots. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3661 (Human); 54131 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human IRF3 aa 206-427 Accession # Q14653 |
| 生物活性 | Human |
| 标记 | Allophycocyanin |
| 背景 | IRF3 (interferon response factor 3) is a 60 kDa member of the IRF family of proteins. Human IRF3 contains one DNA binding domain (aa 7‑107), a nuclear export signal (aa 139‑149) and multiple phosphorylation sites (aa 395‑407). Viral infection stimulates IRF3 phosphorylation, nuclear translocation and stimulation of IFN production. Alternate splice forms may exist. One will show a deletion of aa 201‑327, a second will show the same deletion plus an alternate start site at Met147, and a third will show a 125 aa substitution for the C-terminal 100 aa (aa 328‑427). Over aa 206‑427, human IRF3 is 76% and 83% aa identical to mouse and pig IRF3, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IRF3 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitts lymphoma cell line was stained with Mouse Anti-Human IRF3 APC‑conjugated Monoclonal Antibody (Catalog # IC4019A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
