货号 | FAB1658A |
别名 | GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin-like protein 1; podocalyxin-like; PODXL |
反应种属 | Human |
应用 | Flow Cytometry(10 µL/106cells) |
目标/特异性 | Detects human Podocalyxin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Podocalyxin or recombinant human Endoglycan is observed. |
使用方法 | Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 5420 (Human); 27205 (Mouse) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Mouse myeloma cell line NS0-derived recombinant human Podocalyxin Ser23-Arg427 Accession # AAB61574 |
生物活性 | Human |
标记 | Allophycocyanin |
背景 | Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Podocalyxin in BG01V Human Embryonic Stem Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Podocalyxin APC‑conjugated Monoclonal Antibody (Catalog # FAB1658A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins. |