Human IL-22 Allophycocyanin MAb (Clone 142928) (100 TESTS)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human IL-22 Allophycocyanin MAb (Clone 142928) (100 TESTS)

货号： IC7821A-100 基本售价： 4947.3 元规格： 100 Tests

产品信息

概述
货号	IC7821A-100
别名	Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18
全称	Interleukin 22
反应种属	Human
应用	Intracellular Staining by Flow Cytometry
目标/特异性	Detects human IL-22 in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL‑22 and recombinant rat IL‑22 and no cross-reactivity with recombinant human IL‑10.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	50616 (Human); 50929 (Mouse); 500836 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mycobacterium tuberculosis-Specific IL-21+IFN-gamma+CD4+ T Cells Are Regulated by IL-12. Authors: Li L, Jiang Y, Lao S, Yang B, Yu S, Zhang Y, Wu C PLoS ONE, 2016;11(1):e0147356. Species: Human Sample Type: Whole Cells Application: Flow CX(3)CL1 (fractalkine) and its receptor CX(3)CR1 regulate atopic dermatitis by controlling effector T cell retention in inflamed skin. Authors: Staumont-Salle D, Fleury S, Lazzari A, Molendi-Coste O, Hornez N, Lavogiez C, Kanda A, Wartelle J, Fries A, Pennino D, Mionnet C, Prawitt J, Bouchaert E, Delaporte E, Glaichenhaus N, Staels B, Julia V, Dombrowicz D J Exp Med, 2014;211(6):1185-96. Species: Human Sample Type: Whole Cells Application: Flow Initiation of ART during early acute HIV infection preserves mucosal Th17 function and reverses HIV-related immune activation. Authors: Schuetz A, Deleage C, Sereti I, Rerknimitr R, Phanuphak N, Phuang-Ngern Y, Estes J, Sandler N, Sukhumvittaya S, Marovich M, Jongrakthaitae S, Akapirat S, Fletscher J, Kroon E, Dewar R, Trichavaroj R, Chomchey N, Douek D, O Connell R, Ngauy V, Robb M, Phanuphak P, Michael N, Excler J, Kim J, de Souza M, Ananworanich J PLoS Pathog, 2014;10(12):e1004543. Species: Human Sample Type: Whole Cells Application: Flow IL-22 suppresses IFN-gamma-mediated lung inflammation in asthmatic patients. Authors: Pennino D, Bhavsar P, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham S, Cavani A, Eyerich K, Chung K, Schmidt-Weber C, Eyerich S J Allergy Clin Immunol, 2013;131(2):562-70. Species: Human Sample Type: whole cells Application: Flow Rapid antigen processing and presentation of a protective and immunodominant HLA-B*27-restricted hepatitis C virus-specific CD8+ T-cell epitope. Authors: Schmidt J, Iversen A, Tenzer S, Gostick E, Price D, Lohmann V, Distler U, Bowness P, Schild H, Blum H, Klenerman P, Neumann-Haefelin C, Thimme R PLoS Pathog, 2012;8(11):e1003042. Species: Human Sample Type: Whole Cells Application: Flow Interleukin-21 induces the differentiation of human Tc22 cells via phosphorylation of signal transducers and activators of transcription. Authors: Liu Y, Yang B, Ma J, Wang H, Huang F, Zhang J, Chen H, Wu C Immunology, 2011;132(4):540-8. Species: Human Sample Type: Whole Cells Application: Flow ESAT-6- and CFP-10-specific Th1, Th22 and Th17 cells in tuberculous pleurisy may contribute to the local immune response against Mycobacterium tuberculosis infection. Authors: Qiao D, Yang BY, Li L, Ma JJ, Zhang XL, Lao SH, Wu CY Scand. J. Immunol., 2011;73(4):330-7. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human IL-22 Ala34-Ile179 Accession # Q9GZX6
生物活性	Human
标记	Allophycocyanin
背景	Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.
运输条件	Blue Ice
存放说明	4℃
参考文献	Dumoutier, L. et al. (2000) J. Immunol. 164:1814. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

参考图片

Detection of IL‑22 in Human PBMCs stimulated to induce Th17 Cells by Flow Cytometry.CD4⁺ Human peripheral blood mononuclear cells (PBMCs) either (A) stimulated to induce Th17 cells with 10 ug/mL Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100), 5 ug/mL Mouse Anti-Human CD28 Monoclonal Antibody (Catalog # MAB342), 10 ng/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B), 20 ng/mL Recombinant Human IL‑2 (Catalog # 202-IL), 20 ng/mL Recombinant Human IL‑23 (Catalog # 1290-IL), 40 ng/mL Recombinant Human IL‑6 (Catalog # 206-IL), and 10 ng/mL Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) for 5 to 7 days, then re-stimulated with 50 ng/mL PMA and 200 ng/mL Calcium ionomycin for 2 to 4 hours or (B) resting CD4⁺ PBMCs were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) and Mouse Anti-Human IL‑22 APC‑conjugated Monoclonal Antibody (Catalog # IC7821A). Quadrant markers were set based on isotype control (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.