| 货号 | IC9390T-100 |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | Apg8b; ATG8F; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; lc3b autophagy marker; LC3B; lc3-i, ii; LC3II; MAP1 light chain 3-like protein 2; MAP1A/1BLC3; MAP1A/MAP1B LC3 B; MAP1LC3B; microtubule associated protein 3 b; microtubule associated protein 3 beta; microtubule-associated protein 1 light chain 3 betaMAP1A/MAP1B light chain 3 B; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3B | 全称 | Microtubule-associated Protein 1 Light Chain 3 beta |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human LC3B in flow cytometry. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 81631 (Human); 67443 (Mouse); 64862 (Rat) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | Human LC3B synthetic peptide Accession # Q9H492 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 594 |
| 背景 | Human Microtubule-associated Protein (MAP) Light Chain 3 (LC3) A is a121 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the LC3 subfamily of Autophagy-related 8 (Atg8) proteins (1). The LC3 subfamily also includes LC3B andLC3C. LC3 exhibits 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast autophagy-related protein Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. LC3 was originally described as part is part of a complex that includes heavy and light chains comprising the MAP1 family of microtubule regulatory proteins (3). However, LC3 has gained attention for MAP1-independent functions in autophagy. LC3 utilizes a ubiquitin-like conjugation system that includes E1-, E2-, and E3-like enzymes to covalently attach phosphatidylethanolamine (PE) to its C-terminus, incorporating it into the phagophore membrane during the early stages of autophagasome formation (4). Recruitment of LC3 to the phagophore may promote membrane elongation (4,5). It may also be involved in cargo recruitment to autophagosomes (1). LC3 is often used as a marker of autophagy. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of LC3B in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line either treated with 50 μM chloroquine for 24 hours (filled histogram) or untreated (open histogram) was stained with Rabbit Anti-Human LC3B Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # IC9390T). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |
