| 货号 | FAB362A |
| 别名 | CHEMERINR; chemokine receptor-like 1; chemokine-like receptor 1; CHEMR23; cmklr1; Dez; Gpcr27; G-protein coupled receptor ChemR23; G-protein coupled receptor DEZ; MGC126105; MGC126106; orphan G-protein coupled receptor, Dez | 全称 | Chemokine-like Receptor 1/Orphan G Protein-coupled Receptor ChemR23 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human ChemR23. Stains human ChemR23-transfected cells but not irrelevant transfectants. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1240 (Human); 14747 (Mouse); 60669 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. ChemR23, the receptor for chemerin and resolvin E1, is expressed and functional on M1 but not on M2 macrophages. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0 mouse myeloma cell line transfected with human ChemR23 Met1-Leu371 Accession # NP_004063 |
| 生物活性 | Human |
| 标记 | Allophycocyanin |
| 背景 | ChemR23, also known as CMKLR1, is a 47-48 kDa 7-transmembrane member of the rhodopsin, or G-protein coupled receptor family 1. It has restricted expression on select cell types that, in primate, include adipocytes, astrocytes, endothelial cells, microglia/macrophages, chondrocytes, monocytes, osteocytes, NK cells, and plasmacytoid plus myeloid dendritic cells. In mouse, would studies show ChemR23 to be expressed by neutrophils and keratinocytes. The universally-agreed on ligand for ChemR23 is Chemerin, a 137 amino acid (aa) non-chemokine product of fibroblasts, platelets, adipocytes and endothelium. The Chermerin:ChemR23 system is suggested to promote chemotaxis for all ChemR23+ cells, and to induce angiogenesis via endothelial cell migration and MMP-2 and -9 activation. There is one alternative splice form that shows a deletion of aa 1 and 2 (NP_004063). Over the extracellular domains of ChemR23, human and mouse share 69% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | |
Detection of ChemR23 in Human Whole Blood Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human IL-3 R alpha/CD123 PE‑conjugated Monoclonal Antibody (Catalog # FAB301P) and either (A) Mouse Anti-Human ChemR23 APC‑conjugated Monoclonal Antibody (Catalog # FAB362A) or (B) Mouse IgG3 Allophycocyanin Isotype Control (Catalog # IC007A). View our protocol for Staining Membrane-associated Proteins. |
