| 货号 | FAB812A |
| 别名 | CD135 antigen; CD135; EC 2.7.10; fetal liver kinase 2; FL cytokine receptor; FLK2; Flk-2; FLT3 receptor tyrosine kinase; FLT-3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Stem cell tyrosine kinase 1; STK-1; STK1EC 2.7.10.1; Tyrosine-protein kinase receptor FLT3 | 全称 | fms-like Tyrosine Kinase 3 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detect human Flt‑3/Flk‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Flt-3, recombinant human (rh) SCF R, rhPDGF R alpha, or rhPDGF R beta is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2322 (Human); 14255 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Flt‑3/Flk‑2 Asn27-Asn541 Accession # AAA18947 |
| 生物活性 | Human |
| 标记 | Allophycocyanin |
| 背景 | The Flt-3 (fms-Like Tyrosine kinase) Receptor, also known as Flk-2 (Fetal Liver Kinase) and Stk-1(Stem cell Tyrosine Kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Mouse Flt-3 has also been cloned and shown to share 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Flt‑3/Flk‑2 in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human Flt‑3/Flk‑2 APC‑conjugated Monoclonal Antibody (Catalog # FAB812A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins. |
