Human B7-2/CD86 PerCP MAb (Clone 37301) (100 TESTS)
货号：
FAB141C-100 基本售价：
4808.5 元 规格：
100 Tests
产品信息
概述| 货号 | FAB141C-100 |
| 别名 | Activation B7-2 antigen; B70; B7-2 antigen; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects human B7‑2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, recombinant mouse B7-2, recombinant rat B7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b, rhB7-H4, or rhB7-L2 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 942 (Human); 12524 (Mouse); 56822 (Rat) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. High Cellular Monocyte Activation in People Living With Human Immunodeficiency Virus on Combination Antiretroviral Therapy and Lifestyle-Matched Controls Is Associated With Greater Inflammation in Cerebrospinal Fluid
Authors: T Booiman, FW Wit, I Maurer, D De Frances, CA Sabin, AM Harskamp, M Prins, P Garagnani, C Pirazzini, C Franceschi, D Fuchs, M Gisslén, A Winston, P Reiss, NA Kootstra,
Open Forum Infect Dis, 2017;4(3):ofx108.
Species: Human
Sample Type: Whole Cells
Application: Flow |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑2/CD86 |
| 生物活性 | Human |
| 标记 | PerCP |
| 背景 | B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through Interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Human B7-2 is a 329 amino acid (aa) protein containing a putative 23 aa signal peptide, a 224 aa extracellular domain, a 21 aa transmembrane domain, and a 61 aa cytoplasmic domain. Human B7-2 and B7-1 share 26% amino acid identity. Human and mouse B7-2 share 50% amino acid identity. However, it has been observed that both human and mouse B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Azuma, M.et al. (1993) Nature 366:76.
- Freeman, G.J.et al. (1993) Science 262:909.
- Freeman, G.et al. (1991) J. Exp. Med. 174:625.
- Selvakumar, A.et al. (1993) Immunogenetics 38:292.
- Chen, C.et al. (1994) J. Immunol. 152:4929.
- Freeman, G.J.et al. (1993) J. Exp. Med. 178:2185.
|
参考图片Detection of B7‑2/CD86 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human B7‑2/CD86 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB141C) and Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # IC002C). View our protocol for Staining Membrane-associated Proteins. |
